Current HDAC assay kits have been based on a short wavelength substrate
whose detection is darkened by the presence of autofluorescence interference.
SensoLyte® has ushered in the dawn of ultra-sensitivity.
AnaSpec, EGT Group’s SensoLyte® 520 HDAC Activity
Assay Kit is the industry’s longest wavelength assay kit for HDAC detection
(Ex/Em = 490 nm/520 nm). With a longer wavelength, the SensoLyte®520
HDAC Activity Assay Kit avoids the autofluorescence interference from cell components
and test compounds that is commonly found in traditional HDAC assay kits.
Histone deacetylase (HDAC) enzymes modulate gene expression through
the deacetylation of lysine residues on histone proteins and act as transcriptional
repressors of genes. Based on their role in cell cycling, apoptosis and differentiation,
HDACs have been chosen as therapeutic targets for the treatment of cancer and
The SensoLyte® 520 HDAC Activity Assay Kit provides
a convenient, two-step homogeneous procedure for measuring HDAC activity using
a cell-permeable fluorogenic substrate. Without a time-consuming cell extraction
step, this assay can be used to measure HDAC activity directly in cell culture
in a 96-well plate. This kit can also be used for high throughput screening
of HDAC inhibitors using extracts or purified enzymes.
The kit contains: • HDAC 520 substrate • Deacetylated reference
standard for calibration • HeLa nuclear extract • Assay buffer • Cell lysis
buffer • HDAC developer • Trichostatin A (HDAC inhibitor)
Figure 1. Signal to background ratio comparison of the SensoLyte®
520 HDAC assay kit with the SensoLyte® 440 HDAC assay kit.
Figure 2. Trichostatin A inhibition of HDAC activity in HeLa nuclear
extract as measured with the long wavelength SensoLyte® 520 HDAC Activity Assay Kit.
AnaSpec also offers the SensoLyte® 440 HDAC Activity
Assay Kit as a cost-effective solution for moderate sensitivity. This kit
provides a convenient, two-step homogeneous procedure for measuring HDAC activity
using an AMC-based fluorogenic substrate (Ex/Em=354 nm/442 nm).
To view an HDAC poster presented at a scientific conference,
please click, Development of
Histone Deacetylase Activity Assay Using a Novel Long Wavelength Fluorogenic