5-mC (33D3) & 5-hmC (4D9) - Specific mAbs for Epigenetics Research

5mC & 5hmC mAbs

Methylation of cytosine to 5-methylcytosine (5-mC) is a major epigenetic event which interferes with the binding of proteins to DNA resulting in the exquisite control of gene expression and cell differentiation.

5-mC can be further modified to 5-hydroxymethyl Cytosine (5-hmC) by the TET family of methyl-cytosine dioxygenases. 5-hmC is an oxidative product of 5-mC that appears to participate in several active DNA demethylation pathways. 5-hmC is a key marker related to pluripotency in embryonic stem cells and in cancer cells. Hence, this event may be important for normal processes, as well as during cancer cell development and progression.

AnaSpec is pleased to announce the availability of a mouse monoclonal antibody specifically recognizing 5-hydroxymethyl Cytosine (5-hmC). This monoclonal antibody (Clone 4D9) is available in a 100 µg size (Cat# BI-HMCY-0100). Alternatively, a starter kit (Cat# BI-KMCY-0050) containing 50 µg each of the monoclonal antibody against 5-hmC (Clone 4D9) and the popular 5-mC (Clone 33D3) is also available. Together, these two monoclonal antibodies provide a fully qualified and guaranteed antibody pair for analyzing cytosine methylation pattern in Epigenetics research.

cytosine structure Cytosine
5mC structure 5hmC structure
5-Methylcytosine (5-mC) 5-Hydroxymethyl Cytosine (5-hmC)

Monoclonal Antibody against 5-Hydroxymethylcytosine (5-hmC)
Figure 1.Dot blot of 5-hmC mAb (4D9) with DNA standard, Cytosine (C), 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC).
Figure 2. Titer of 5-hydroxymethylcytosine (5-hmC) monoclonal antibody (4D9). Estimated titer is 0.05 µg/ml.

Anti-Hydroxymethyl Cytosine (5-hmC) mAb Anti-Methyl Cytosine
(5-mC) mAb
5-mC & 5-hmC mAb
Starter Kit
Catalog# BI-HMCY-0100 (0.1 mg)

BI-MECY-0100 (0.1 mg)

BI-MECY-0500 (0.5 mg)

BI-MECY-1000 (1 mg)

BI-KMCY-0050 (0.05 mg)
Clone # 4D9 33D3
50 µg each:
5-mC mAb (33D3)
5-hmC mAb (4D9)
Host Mouse Mouse
Isotype IgG1 IgG1λ
Species Reactivity All species All species
Specificity 5-hmC 5-mC
Concentration 1 mg/ml in PBS 1 mg/ml in PBS

Epigenetics Products:

Histone Peptides
AnaSpec offers a wide selection of GO® (catalog) Histone peptides (H1, H2A, H2B, H3 & H4), making it one of the world's largest providers of Histone peptides for the Epigenetics research community.

AnaSpec's histone sequences include:

  • Lysines
    • Acetylated
    • Mono-, di- , tri-methylated
    • Crotonylated
    • Ubiquitinated
  • Arginines
    • Mono-methylated, a/symmetrically di-methylated
    • Citrullinated
  • Serines or Threonines
    • Phosphorylated
  • Sequences with or without a biotin tag

Please click below to view the full listing of Histone peptides. Contact biology@anaspec.com to obtain help to look up a certain peptide.

Histone H1 Peptides
Histone H2A Peptides
Histone H2B Peptides
Histone H3 Peptides
Histone H4 Peptides
Histone Related Substrates

Assay Kits

SensoLyte® 520 HDAC Assay Kit
SensoLyte® 520 Deubiquitination Assay Kit
SensoLyte® 440 Deubiquitination Assay Kit
SensoLyte® 520 FRET SIRT1 Assay Kit
SensoLyte® Green SIRT1 Assay Kit
SensoLyte® 520 FRET SIRT2 Assay Kit
SensoLyte® Green SIRT2 Assay Kit
SensoLyte® HAT (p300) Assay Kit

Unusual Amino Acids

PTM Lysines & Arginines for synthesis of PTM containing peptides