EnzoLyte Plus MMP Assay Kits Offer Focused Selectivity for Identifying Specific MMPs

Focused MMP Detection

Current EnzoLyte™ MMP assay kits can sensitively detect the activity of MMPs but have been limited in their specificity by the cross-reactions between different MMPs. AnaSpec presents an innovative solution for the identification of specific MMPs. Matrix Metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components1;2. These enzymes are responsible for connective tissue deterioration, bone remodeling, and damaged tissue repair. They are also involved in a number of diseases, such as tumor development and metastasis3-6 as well as in rheumatoid arthritis7;8. EnzoLyte Plus™ MMP assay kits are the ideal solution for researchers who need to identify the activity of a specific MMP in a complex biological sample containing multiple MMPs and other peptidases. EnzoLytePlus™ MMP Assay Kits integrate the specificity of a monoclonal antibody with the sensitivity of a fluorescence peptide substrate, detecting the presence of a particular MMP in biological samples, including serum, plasma, tissue homogenate, cell lysate, synovial fluid and more.

The enhanced selectivity is possible through the inclusion of a monoclonal anti human-MMP coated 96-well plate (12 x 8 black strips). The antibody specifically captures the proenzyme and active forms of an MMP from a mixed biological sample. The wells are washed and subsequent addition and cleavage of the FRET peptide gives a fluorimetric reading that reflects the proteolytic activity of the specifically captured MMP. AnaSpec’s unique 5-FAM/QXLTM 520 FRET peptides provide highly sensitive fluorescence readouts at an emission wavelength of 520 nm with a convenient excitation of 490 nm. Experimental data confirms that these kits can measure subnanogram quantities of specific human MMPs, with no cross-reaction with other MMPs.

AnaSpec currently offers the following MMP-specific assay kits:

EnzoLyte Plus™ 520 MMP-1 Assay Kit
EnzoLyte Plus™ 520 MMP-9 Assay Kit
EnzoLyte Plus™ 520 MMP-13 Assay Kit

To view all EnzoLyteTM MMP Assay Kits on-line, click here

To request the 2006-2007 Detection Reagents and Kits catalog, click here

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2. Woessner, JF. Jr., FASEB J. 5, 2145 (1991).
3. Matrisian, LM. et al., Proc. Natl. Acad. Sci. U.S.A 83, 9413 (1986).
4. Collier, IE. et al., J. Biol. Chem. 263, 6579 (1988).
5. Salo, T. et al. J. Biol. Chem. 258, 3058 (1983).
6. Salo, T. et al. J. Biol. Chem. 260, 8526 (1985).
7. Chin, JR.et al. J. Biol. Chem. 260, 12367 (1985).
8. Okada, Y.et al. J. Biol. Chem. 261, 14245 (1986).