Parkinson's & Alzheimer's Disease Products
Alpha Synuclein>
Parkinson & Alzheimer's
Diseases

AnaSpec offers a range of Synuclein products - Assay, Recombinant Proteins, and Antibodies for research use.

α -Synuclein(SNCA) and β -Synuclein (SNCB), are expressed primarily in neural tissue and in some tumors (read more).

Assay Kit Benefits & Results

One Step Convenient Format

➢ Pre-coated/blocked strip plate

➢ Ready-to-use solutions

➢ One step assay

➢ 1 hour assay time
(excluding incubation time)

Minimal Sample Size

➢ Requires only 10-20 μl of cell and tissue lysate or body fluids.

➢ Detects 5 pg/ml of
α-Synuclein

Broad Dynamic Range

➢ 5-500 pg/ml α-Synuclein in the assayed sample
Recombinant Protein Benefits

QC tests
➢ >95% by HPLC
➢ Cat# AS-55555 is tested for fibrillization

Special Options
➢ Fluorescent & Biotin labeled versions
➢ Low Endotoxin version available

Special Conjugations
➢ Custom conjugations to Fluorescent dyes, Europium, QXL-Quenchers, etc.
PRODUCTS
Synuclein ELISA Kit Dynamic Range Detection Limit Main kit contains Assay time Number
of Assays in
96-well plate
Catalog Number
SensoLyte® Anti-α-Synuclein (Human) ELISA Kit
8-500 pg/ ml 5 pg Anti-α-Synuclein 8-well strip plate (8x12) 2-3 hours 96 assays

AS-55550-H

Synuclein Recombinant Proteins Catalog Number Size
EndoClear™
Recombinant α-Synuclein (1 - 140)

AS-55555-100
AS-55555-500
AS-55555-1000
0.1 mg
0.5 mg
1 mg
Recombinant α-Synuclein (1 - 140), biotin labeled
AS-55581
0.2 mg
Recombinant α-Synuclein (1 - 140), HiLyte™ Fluor488 labeled

AS-55457
0.2 mg
Recombinant β-Synuclein (1 - 134)
AS-55458-100
AS-55458-500
AS-55458-1000
0.1 mg
0.5 mg
1 mg
DJ-1 (PARK7) Protein DJ-1 (PARK7) Protein, GST tagged
Synuclein Antibodies Catalog Number Size
Anti-α-Synuclein (pSer 87), polyclonal
AS-55329
50 μg

About Synuclein

α -Synuclein(SNCA) and β-Synuclein (SNCB) proteins, along with γ-synuclein (SNCG), are members of the Synuclein family of small proteins expressed primarily in neural tissue and in some tumors.1 Synuclein proteins, found only in vertebrates, possess a highly conserved N-terminal domain, with a variable number of 11-residue repeats and a less conserved C-terminal, with a preponderance of acidic residues.1

α-Synuclein
α-Synuclein is an abundant protein of 140 residues that is present in high concentration at presynaptic terminals and is found in both soluble and membrane-associated fractions of the brain. Several possible functions have been suggested, among which are vesicle release and trafficking. In vitro incubation in the presence of salt (i.e. 0.1M NaCl) with agitation causes α-Synuclein to form fibrils.2-6

β-Synuclein
N-terminal of β-Synuclein is highly homologous to α-, γ-Synucleins and consists of degenerative "KTKEGV" repeats.7-10 Similar to α-Synuclein, β-Synuclein is found primarily in the brain. However, it is not associated with Lewy bodies in Parkinson disease like α-Synuclein.7-10 Beta-synuclein was found to inhibit production of phosphatidic acid by the phospholipase D2 transmembrane protein in vitro.7In addition, β-Synuclein was detected in many breast and ovarian tumors.7 Recent investigations demonstrated that β-synuclein can induce mild experimental autoimmune encephalomyelitis (EAE) in Lewis rats.8

RELATED PRODUCTS for AD Research

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Assay Kits & Dyes

References:
1. George, JM. Genome Biol. 3, reviews 3002.1 (2002).
2. Trojanowski, JQ. & VM. Lee, Ann. N. Y. Acad. Sci. 991, 107 (2003).
3. Masliah, E., et al. Science 287, 1265 (2000).
4. Van Der, P. H, et al. J. Neurosci. 20, 6021 (2000).
5. Feany, MB. & WW. Bender, Nature 404, 394(2000).
6. Weinreb, P. H., et al. Biochemistry 35, 13709 (1996).
7. Rivers, R.C. et al. Protein Sci 17, 887 (2008).
8. Sung, Y-H. et al. Protein Sci 15, 1162 (2006).
9. George, JM. Genome Biol 3, 3002.1 (2001).
10. Bruening, W. et al. Am Cancer Soc 88, 2154 (2000).
11. Kela-Madar, N. et al. J Neuroimmunol 208, 19 (2009).