Quant-Peptides
Quant Peptides>
Quantitation Peptides

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Quant-Peptides are customized peptides that are accurately quantified. They are intended to be used as standards for quantitative proteomics.

Classical quantitation methods such as Amino Acid Analysis are inherently variable, resulting in standards that are too inaccurate for high precision proteomic assays. Eurogentec has developed innovative technologies that guarantee the accurate quantification of peptide standards. Quant-peptides are available in two formats: without or with a Quant-Tag

 

Benefits
Accurately quantified
Ready-to-use in special glass tubes
Small size dispensing option

Specifications
Light or heavy-isotope labeled peptides
Purity: >85 to >95%
Minimum order amount: 0.5 mg
Dispensing options: e.g. 100 aliquots/mg
Dried or in solution


Two technologies
that offer high precision custom peptide standards, and can be complementary:

Quant-Peptides with no Quant-Tag* are absolutely quantified using a proprietary and highly optimised AAA-MS method. These are universal peptide standards which can be used in any proteomics application.
quant_peptide
Quant-Peptides with a Quant-Tag** are absolutely quantified based on the inherent spectral properties of the proprietary tag. The Quant-Tag is coupled to the C-term of the peptide via an Arginine (R) or Lysine (K) residue and can be released by trypsin digestion. The precise molecular mass of the tag (1356,7 Da) can be used in assessing the cleavage efficacy, and hence in setting the optimal trypsinization conditions of a sample using i.e. MS:MS methods. To do so, the Quant-Peptide must be spiked into the sample prior to trypsin digestion. The Quant-Tag is then used as a reporter of the trypsin digestion efficacy : a peak corresponding to the tag mass is indicative of trypsin digestion.
quant tag
Applications:
Quantitative Proteomics,
High-Throughput Assays
Absolute quantification of protein-based biomarkers from complex samples is central to pharmaceutical and diagnostic proteomics.

The identification and quantification of peptides derived from a protein of interest, is routinely determined by employment of several mass spectrometry based methods, including Single/ Multiple Reaction Monitoring (SRM/MRM). A prerequisite for such measurements, is the availability of accurately quantified peptide standards used for internal calibration during mass spectrometry. Quant-Peptide standards offer this solution.
Option:
Dispensing Quant-Peptides
In quantitative proteomic analyses, using identical amounts of peptide standard in each sample is mandatory, as this spiked peptide is the calibrator from which the peptide of interest, present in the sample as trace amount, is quantified. Therefore, Quant-Peptides are dispensed in a highly reproducible and controlled manner to ensure assay reproducibility and accuracy.
  • Accurate quantities & no peptide waste
  • Long shelf life of the individual aliquots
  • Ready-to-use with no manual aliquoting required
  • Vial to vial accuracy & reproducibility results from our highly controlled process
  • Direct Access to the right quantity; no peptide waste
quantpeptides

Contact
To start discussing your project or receive a quote, please contact us at:
For US: chemistry@anaspec.com
For EU: proteomics.services@eurogentec.com