HCV NS3/4A Protease FRET Substrates


HCV NS3/4A - Ultra Sensitive

NS3/4A protease is the protease responsible for the cleavage of HCV nonstructural polyprotein at the NS3-NS4A, NS4A-NS4B, NS4B-NS5A, and NS5A-NS5B sites. Since these cleavages are essential for the maturation of the viral proteins, this protease has become one of the key targets for developing anti-HCV drugs. Focusing on this particular protease, AnaSpec has developed an innovative new tool for optimized detection performance.

Applying both peptide and fluorescent dye technologies, AnaSpec has developed an ultra-sensitive HCV NS3/4A protease FRET substrate that can detect the presence of HCV NS3/4A protease at levels of < 0.1 pmol. Based on the peptide sequence, Ac-DED(EDANS)EE-Abu-[ψ,COO]ASK(DABCYL)-NH2, developed by Taliani, M. et al.1, the donor and the quencher have been substituted with 5-FAM and our trademark quencher QXLTM 520, respectively. This substrate, Ac-DE-Dap(QXL520)-EE-Abu-[ψ,COO]AS-C(5-FAMsp)-NH2, shows a 22-fold lower Km than the EDANS/DABCYL peptide.

To view the entire comparison data from the poster presented by our R& D scientists at the 2005 Viral Hepatitis Meeting, please click here. To request a hard copy, Click here.

Hydrolysis products for these two substrates are available for use as experimental controls.

In addition to the HCV Protease substrates, AnaSpec actively supports HCV research by supplying a number of different HCV non-structural (NS) protein fragments. To view, click here.

Click here to request AnaSpec's newest 2006-2008 Peptides Catalog

References:
1. Taliani, M. et al., Anal.Biochem. 240, 60-67 (1996).