EnzoLyte HCV Assay Kits - Industry Leader

EnzoLyte HCV Assay Kits - Industry Leader

Hepatitis C virus (HCV), belonging to the Flaviviridae family of positive, single stranded RNA, infects approximately 170 million people worldwide1. The HCV polyprotein consists of structural proteins (C, E1, E2 and p7), and the non-structural (NS) proteins (NS2, NS3, NS4A, NS4B, NS5A, and NS5B) as a result of proteolytical cleavages of host signal peptidases; and metalloprotease and serine proteases, respectively.

AnaSpec is pleased to introduce the industry’s only HTS assay kits for screening HCV NS3/4a. EnzoLyte™ assay kits provide a fast, convenient, and cost-effective solution for the optimized detection of HCV NS3/4a and HIV-1 protease activity at subnanomolar concentrations.

AnaSpec’s EnzoLyte™ HCV Protease Assay Kits are kits specifically designed for the detection and quantification of NS3/4A protease activity. They are convenient, easy-to-use assay kits that can be used in 96- or 384-well microplate format. There are three assay kits to choose from depending on what wavelength is used for monitoring the cleavage of the FRET substrate. Convenient mix-and-read format and one-step procedure make EnzoLyte Assay Kits ideal for high-throughput screening.

EnzoLyte™ 490 HCV Assay KitMost Assays
  • Peptide substrate paired with Dabcyl (fluorophore) and Edans (quencher). Fluorescence can be continuously monitored at Ex/Em=340/490nm. Kit size: 500 assays.

  • EnzoLyte™ 520 HCV Assay KitMost Sensitive
  • Peptide substrate paired with 5-FAM (fluorophore) and QXL™ 520 (quencher). Fluorescence can be continuously monitored at Ex/Em=490/520 nm. Kit size: 100 assays.

  • EnzoLyte™ 620 HCV Assay KitAvoids Autofluorescence Interference
  • Peptide substrate paired with HiLyte™ Fluor TR (fluorophore) and QXL™ 610 (quencher). With near red emission wavelength, this FRET peptide avoids the autofluorescence interference associated with most test compounds. Fluorescence can be continuously monitored at Ex/Em=590/620nm. Kit size: 100 assays.

  • * Highly active recombinant HCV NS3/4A protease available separately - Cat# 61017

    For a full listing of EnzoLyte™ Assay Kits, click here.

    To request the 2006-2007 Detection Reagents & Kits catalog, click here

    1. CDC and Prevention. Morb. Mortal. Wkly. Rep. 47:1-39 (1998).