AnaTag™ Protein Labeling Kits – World-Class Performance Made Easy

The labeling of proteins and antibodies with fluorescent dyes is crucial for researchers who use flow cytometry, immunohistochemistry and fluorescent in-situ hybridization techniques. Incorporating some of the industry's brightest and most photostable dyes in an ultra-fast, ultra-convenient kit, AnaSpec is pleased to showcase its line of AnaTag Protein Labeling Kits – a perfect marriage of performance and convenience.

High-Performance HiLyte™ Fluor Dyes

AnaTag Protein Labeling Kits incorporate AnaSpec's proprietary HiLyte™ Fluor dyes.

HiLyte™ Fluor dyes provide:

  • More intense fluorescence than classic dyes such as FITC, TAMRA, and ROX
  • Greater photostability than FITC, Cy3, and Cy5 dyes.
  • Compared to Cy3 and Cy5, HiLyte™ Fluor 555 and 647-protein conjugates are only slightly red-shifted, resulting in a more optimal match to filters designed for Cy3 and 5 dyes.
  • Recent studies indicate that HiLyte™ Fluor 555 and 647 are superior to Cy3 and Cy5 in fluorescence polarization-based assays.
  • Unlike Cy5 dyes, HiLyte™ Fluor 647 dyes have very little change in absorption or fluorescence spectra when conjugated to most proteins, oligonucleotides and nucleic acids, thus yielding greater total fluorescence at the same degree of substitution.

  • Utilizing both HiLyte™ Fluor and classic dyes, AnaTag™ Protein Labeling span the spectrum from blue to far red and NIR.


    AnaTag Protein Labeling Kits provide a fast and convenient kit for labeling primary and secondary antibodies.

  • Labeling time - as short as 30 minutes
  • All essential components for conjugation reaction and purification of dye-protein conjugates
  • Available in two convenient reaction sizes: Micro-scale labeling kit: 200 ug x 3 reactions or 100 ug x 5 reactions and larger scale labeling kit: 5 mg x 3 reactions
  • Industry-standard amine-reactive chemistry

  • Stable dye-protein conjugate products

    The succinimidyl ester (SE) reactive form of the labeling dye reacts with the amine group on the protein and forms stable carboxamide bonds which are identical to and as stable as the natural peptide bond. These dye?protein conjugates are very stable and are suitable for immunofluorescent staining, fluorescence in situ hybridization, flow cytometry and other biological applications.

    AnaTag Protein Labeling Kits combine world-class performance and convenience without compromising on value. By caring for both the research and budgetary needs of our customers, AnaSpec is perfecting The Science of Trust.

    AnaTagTM Labeling Kits
    Number of Reactions
    Product NameAbs/Em (nm/nm) 3 x 5 mg 3 x 200 ug
    AMCA-X353/442 7205572056
    5-FITC494/519 7205972060
    HiLyte™ Fluor 488499/523 7204772048
    5-TAMRA547/574 7206372064
    HiLyte™ Fluor 555553/568 7204572046
    HiLyte™ Fluor 647652/66972049 72050
    HiLyte™ Fluor 750754/778 72043 72044