Custom Anti-Phosphopeptide Antibodies


Custom Anti-Phosphopeptide Antibodies

For raising antibodies against phosphopeptides there are 3 critical factors for success: (1) the stability of the phosphorylation, (2) the purity of the peptide and (3) post-development purification of the phospho-specific antibody. With documented experience in meeting all three factors, AnaSpec is a proven source for world-class phosphospecific antibody production.

As a trusted provider to the global research community, AnaSpec is experienced in fulfilling the most stringent demands for optimizing project success. Using technologies that are fine-tuned to produce high-yield, ultra-pure phosphopeptides, AnaSpecís peptide chemists routinely synthesize sequences containing single and multiple phosphoamino acids. With full manufacturing facilities located in San Jose, CA, AnaSpec is able to maintain ISO9001:2000 manufacturing processes and robust quality validation.

AnaSpec custom antibodies are produced using an optimized protocol that produces premium quality antibodies on an efficient 11-week schedule.

AnaSpecís Complete Phosphopeptide Antibody Package includes:
  • 15 mg (>90% purity, up to 15 amino acids) phosphospecific peptide - 5 mg delivered to researcher, 10 mg for antibody production.
  • 11 mg (>80% purity, up to 15 amino acids) non-phosphospecific peptide Ė used for affinity depletion (in-house only)
  • Immunization of 2 rabbits
  • Phosphospecific and non-phosphospecific peptide ELISA titration of antibody for all bleeds
  • Pre-immune sample of serum (5 mL/each rabbit) and anti-serum (100 mL total)
  • Affinity purification of selected batch of serum
  • Shipment of purified phosphospecific antibody which does not cross-react with non-phosphospecific peptides (as confirmed by ELISA)

  • Our customers have the added option of ordering non-hydrolyzable phosphoserine-containing peptides (click here for more details).

    Click here to request a quotation.

    Click here to request AnaSpecís 2006-2007 Detection Reagents & Kits catalog


    Select References to AnaSpecís phosphopeptide antibodies:

    Zhou, Q. et al. Keratin 20 serine-13 phosphorylation is a stress and intestinal goblet cell marker. J. Biol. Chem. 10.1074/jbc.M512284200 (2006).

    Tanaka, H. et al. DNA damage-induced phosphorylation of the human telomere-associated protein TRF-2. PNAS 102, 15539 (2005).

    Toivola, DM. et al. Type II keratins are phosphorylated on a unique motif during stress and mitosis in tissues and cultured cells. Mol. Biol. Cell 13, 1857 (2002).