Leveraging its core antibody and dye technologies, AnaSpec
is pleased to introduce a suite of labeled and unlabeled anti-Actin
options include biotin, conventional fluorescent dyes, and AnaSpec's proprietary
line of HiLyte™ dyes.
The NIH/3T3 whole cell lysate (Cat#29515) was probed by Rabbit anti-Actin(Cat.
# 29553) at 1: 200.
||Human Leiomyoma stained with
anti-Actin (Cat# 29553). Formalin-fixed tissues was boiled in 10 mM citrate
buffer, pH 6.0 for 10 min, followed by cooling at RT for 20 min, then
probed at a dilution 1:100 for 10 min at room temperature.
Actin is a highly conserved protein of approximately 42-kDa, expressed
in all eukaryotic cells. Actin filaments are constituents of the
cytoskeleton and play essential roles in regulating cell shape, cell division,
cell junctions, cell signaling, and movement. Actins are divided into three classes - alpha,
beta and gamma. At least six Actin isotypes have been identified in mammalian cells. Alpha
Actins are found in muscle (α-skeletal, α-aortic smooth, α-cardiac,
and γ2-enteric smooth). Beta and gamma isoforms are prominent in non-muscle
cells. Smooth muscle a-Actin is important because its expression
is mostly detected in vascular smooth muscle cells. Anti-Actin antibodies stain smooth muscle cells in vessel walls, gut wall, myometrium, myoepithelial
cells, and is reactive with tumors arising from smooth muscles and myoepithelial