Kinase/Phosphatase Substrates are GO™


As advances in kinase research continue to speed up, researchers are constantly in search of tools that can keep up. With over 300 research-ready kinase/phosphatase substrates, AnaSpec makes sure that your kinase discovery is ready to go.

Protein kinases are a transferase family of self-regulatory enzymes that catalyze the phosphorylation of other proteins by covalently attaching phosphate groups to them, using ATP as a phosphate donor. Reversible protein phosphorylation-dephosphorylation has a principal role in the regulation of essentially all cellular functions and most aspects of cell life, and phosphorylation by protein kinases is a major signal transduction mechanism that regulates gene expression, proliferation, membrane transport, cell metabolism and many other cellular functions by modifiying other proteins.

This month we are pleased to showcase our extensive collection of kinase/phosphatase substrates. These substrates can be found grouped according to their kinase families. A single substrate can have a dizzying number of modifications (see Table 1).

Research moves fast. Kinase/phosphatase substrates from AnaSpec are GO.

To get started finding the kinase/phosphatase substrate you need, just type in the sequence you are looking for into our Smart Search tool.

For peptide array needs, please email your requirements to service@anaspec.com.

Kinase Substrate Categories

AKT/PKB/Rac-protein kinases

Calcineurin (PP2B)

CaMK (Ca2+/calmodulin-dependent protein kinase)

CaMK II (Ca2+/calmodulin-dependent protein kinase II)

CDC2; CDK1 (Cyclin-dependent protein kinase 1)

CDK5 (Cyclin-dependent Kinase 5)

CK I (Casein Kinase I)

CK II (Casein Kinase II)

EGFR (Epidermal Growth Factor Receptor)

Glycogen Synthase

GSK-3 (Glycogen Synthase Kinase - 3)

GSK-3β (Glycogen Synthase Kinase - 3β)

Insulin Receptor Tyrosine Kinase

Kinase Substrate Library

MAP kinases

Myristoylated Alanine-rich C-Kinase

P60c-src

PKA (Protein Kinase A)

PKC (Protein Kinase C)

PKG (Protein Kinase G)

Protein Tyrosine Kinases

PTP (Protein Tyrosine Phosphatase)

PTP 1B (Protein Tyrosine Phosphatase 1B)

S6 Kinases

Tyrosine Hydroxylase

Tyrosine Kinase Peptide 1

Tyrosine Kinase Peptide 3

Table 1. Modifications of Kemptide (Leu-Arg-Arg-Ala-Ser-Leu-Gly)

LRRASLG

Native

NA

771.9

1 mg

5 mg

25 mg

22593

22594

22595

5-FAM-LRRASLG

Green fluorescent

494/522

1130.2

1 mg

5 mg

29933-1

29933-5

5-TMR-LRRASLG

Red fluorescent

546/574

1184.4

1 mg

5 mg

29934-1

29934-5

Biotin-LRRASLG

Biotinylated

NA

998.2

1 mg

5 mg

25 mg

60527

24533

24534

Biotin-LC-LRRASLG

Biotinylated-LC

NA

1110.4

5 mg

25 mg

24535

24536

HiLyte™ Fluor 488-LRRASLG

Green fluorescent

498/525

1128.3

1 mg

61233

LRRASVA

[Val6, Ala7] Kemptide

NA

771.9

5 mg

25 mg

22596

22597

LRRWSLG

[Trp4] Kemptide

NA

887.1

5 mg

25 mg

22598

22599

LRRAALG

Negative Control

NA

755.9

1 mg

5 mg

24428

24429

LKRA-pY-LG-NH2

[Lys2, pTyr5] Kemptide

NA

899.0

1 mg

5 mg

20290

24528

LKRA-pT-LG

[Lys2, pThr5] Kemptide

NA

837.0

1 mg

5 mg

20295

24497

LRRA-pS-LG

Phosphorylated

NA

851.9

1 mg

5 mg

60528-1

60528-5

Biotin-LRRA-pS-LG-NH2

Biotinylated, Amidated

NA

1077.2

1 mg

5 mg

60565-1

60565-5

Biotin-LC-LRRA-pS-LG

Biotinylated with LC

NA

1191.4

1 mg

5 mg

60566-1

60566-5

Biotin-LRRA-pS-LG

Biotinylated

NA

1078.2

1 mg

5 mg

60567-1

60567-5

HiLyte™ Fluor488-

LRRA-pS-LG

Green fluorescent

498/525

1208.3

1 mg

61234

5-TMR-LRRA-pS-LG

Red fluorescent

NA

1264.4

1 mg

60467-1