AnaSpec is pleased to announce the release of the industry's first
fluorimetric assay optimized to detect ADAMTS-4 (aggrecanase-1) activity -
the SensoLyte™ 520 Aggrecanase-1 Assay Kit. A FRET based assay, it
is homogeneous and has an emission in the green range (Ex/Em= 490 nm/520 nm).
The kit contains a novel internally quenched peptide substrate for aggrecanase-1
consisting of 5-FAM/TAMRA as the FRET pair. In the intact FRET peptide, the
fluorescence of 5-FAM is quenched by TAMRA. Active ADAMTS4 cleaves FRET substrate
into two separate fragments resulting in an increase of 5-FAM fluorescence.
Other members of aggrecanase family, such as ADAMTS-1 and ADAMTS-5, do not
cleave the substrate provided in this kit (Figure 1).
are members of the ADAMTS (A Disintegrin And Metalloprotease with Thrombospondin
motif) family of proteases. ADAMTS-4 (Aggrecanase-1) and ADAMTS-5 (Aggrecanase-2)
activities result in the degradation of cartilage aggrecan in arthritic diseases.1-3
ADAMTS-4 (aggrecanase-1) is a major aggrecanase in human osteoarthritic cartilage.4
Other members of the ADAMTS family (ADAMTS-1, -8, -9, -15 and –16) have also
been implicated in embryonic development and angiogenesis.5
The kit provides enough reagents to perform 100 assays in a
contains: • Aggrecanase-1 FRET substrate • 5-FAM reference standard for calibration
• Assay buffer • Aggrecanase inhibitor • Stop Solution.
Click here to
request more information.
1. The substrate in the SensoLyte™ 520 Aggrecanase Assay Kit shows
efficient cleavage by ADAMTS-4, and negligible cleavage by ADAMTS-1 or
Recombinant Aggrecanase Proteins
WAAG-3R, Aggrecanase (ADAM-TS-4) FRET Substrate
490 MMP Assay Kits - fluorimetric
MMP ELISA Assay Kits - colorimetric
PK. et al. J. Clin. Invest. 1059, 1335 (2000).
MD. Science 284, 1663 (1999).
J. Clin. Exp. Rheumatol. 26, 139 (2008).
S, et al. Pathol. Int. 57, 703 (2007).
BL. Int. J. Biochem. Cell Biol. 33, 33 (2001).
is the industry's sole provider of long-wavelength Aggrecanase assay kits.