AnaSpec, known for developing sensitive, long wavelength assays, is pleased
to announce the release of another long wavelength protease assay – the SensoLyte®
Rh110 Plasmin Activity Assay Kit (Ex/Em=496 nm/520 nm). A shorter
wavelength plasmin assay, the SensoLyte® AFC Plasmin Activity Assay Kit (Ex/Em=380
nm/500 nm) is also released.
is a serine protease derived from the conversion of plasminogen in blood plasma
by plasminogen activators. Its primary function is to degrade fibrin
in blood clots.1 Plasmin activators and inhibitors are thought to
be the key participants in the balance between proteolytic and antiproteolytic
activities that regulate extracellular matrix degradation.2 In addition
to its established role in fibrinolysis, plasmin is also involved in several
pathological and physiological processes such as embryogenesis, ovulation, inflammation,
wound healing, angiogenesis, neoplasia, and metastasis.2
The SensoLyte® Rh110 Plasmin Assay Kit provides
a convenient assay for screening of enzyme inhibitors and activators or for
continuous assay of enzyme activity using a fluorogenic substrate. Upon plasmin
protease cleavage, this substrate generates the Rh110 (rhodamine 110) fluorophore
which has a bright green fluorescence and can be detected at Ex/Em=496 nm/520
nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110
provide greater sensitivity and less interference from other reaction components.
Figure 1. Detection of plasmin with the SensoLyte®
Rh110 Plasmin Activity Assay Kit. The detection limit can reach as low as
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1. Collen, D. Circulation, 93, 857 (1996).
2. Vassalli, J.D. et al, J. Clin. Invest. 88, 1067 (1991).