SensoLyte® Luminescent Peroxidase Assay Kits – NEW


Peroxidases are a group of heme­-containing enzymes that catalyze oxidation-reduction reactions. Hydrogen peroxide (H2O2) or lipid peroxides are the substrates used by peroxidases for a number of oxidative reactions. Peroxidases are found in bacteria, fungi, plants and animals. Cellular peroxidases play an important role in protecting cell from oxidative injury. Horseradish peroxidase (HRP) is extensively used in ELISA and IHC as a reporter molecule when conjugated with secondary detection reagents.

AnaSpec is pleased to introduce two luminescent read-out assay kits for determining peroxidase activity – the SensoLyteŇ Luminescent Peroxidase Assay Kit and the SensoLyteŇ Luminescent Peroxidase ELISA Assay Kit. The former detects peroxidase activity in biological samples and in ELISA with HRP-conjugates while the latter detects HRP conjugated with antibody or streptavidin in ELISA. In both assays, the chemiluminescent substrate luminol is oxidized by peroxide and creates an excited state intermediate product that decays to a lower energy state by releasing photons of light.

The kits provide ample materials to perform 500 assays in a 96-well format. The protocol can readily be modified to run assays in a 384-well format.

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Figure 1. Detection of horseradish peroxidase with the SensoLyte
Ň Luminescent Peroxidase Assay Kit. The detection limit can reach femtogram range (n=3, mean±S.D).


Figure 2. The SensoLyte
Ň Luminescent Peroxidase ELISA Assay Kit was used to detect goat anti-rabbit biotinylated antibody. Wells were coated with total rabbit IgG. After blocking and washing, serially diluted goat anti-rabbit antibody conjugated with biotin was added into the wells, followed by incubation with HRP-labeled streptavidin. The plate was washed again and the peroxidase substrate mixture added to wells. Signal was immediately measured (Flexstation 384II, Molecular Devices). The assay can detect as low as 1 pg of biotinylated goat-anti-rabbit antibody.


Product

Size

Catalog #

SensoLyte® Luminescent Peroxidase Assay Kit *Luminometric* NEW

1 kit

72127

SensoLyte® Luminescent Peroxidase ELISA Assay Kit *Luminometric* NEW

1 kit

72128


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