HiLyte™ Fluor 594 Labeled Secondary Antibodies & Streptavidin


AnaSpec is pleased to offer secondary antibodies and streptavidin conjugated to the newest member of the HiLyte™ Fluor family - HiLyte™ Fluor 594 (Ex/Em=593/616 nm). Using optimal fluorophore/protein labeling ratio, these HiLyte™ Fluor 594-IgG conjugates exhibit high fluorescent signal and uncompromised biological function. The fluorescence of HiLyte™ Fluor 594 can be detected with filters designed for Texas Red® (Invitrogen).

The labeling performance and stability properties of HiLyte™ Fluor 594 are better than those of Texas Red®. It has a higher extinction coefficient (80,000 M-1cm-1) and a lower correction factor (0.17) compared to Alexa Fluor™ 594 (Invitrogen). The fluorescence quantum yield of HiLyte™ Fluor 594 is 0.9 in aqueous solution. Biomolecules conjugated to HiLyte™ Fluor 594 exhibit little spectral overlap with green-fluorescent conjugates, and can be efficiently excited by 568 nm line of Ar-Kr laser and by the 594 nm line of orange He-Ne laser. HiLyte™ Fluor 594 conjugated streptavidin provides high fluorescence intensity and low background as validated in immunofluorescence staining of mammalian cells. HiLyte™ Fluor 594-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications.

The newly developed HiLyte™ Fluor 594 is available in a variety of reactive forms, such as acid, amine, C2 maleimide, hydrazide, succinimidyl ester (SE).

Product

Size

Catalog #

Goat anti-rabbit IgG (H+L), HiLyte™ Fluor 594 labeled

500 µg

28176-H594

Goat anti-rabbit IgG (H+L), highly cross-adsorbed, HiLyte™ Fluor 594 labeled

500 µg

61056-H594

Goat anti-mouse IgG (H+L), HiLyte™ Fluor 594 labeled

500 µg

28175-H594

Goat anti-mouse IgG (H+L), highly cross-adsorbed, HiLyte™ Fluor 594 labeled

500 µg

61057-H594

Rabbit anti-mouse IgG (H+L), HiLyte™ Fluor 594 labeled

500 µg

28164-H594

Rabbit anti-goat IgG (H+L), HiLyte™ Fluor 594 labeled

500 µg

28168-H594

Rabbit anti-chicken IgY (H+L), HiLyte™ Fluor 594 labeled

500 µg

29709-H594

Streptavidin, HiLyte™ Fluor 594 conjugated

1 mg

60672-H594

Related Products:

Anti-Actin (Smooth muscle specific) HiLyte™ Fluor 594-labeled NE
Anti-GST Tag HiLyte™ Fluor 594 labeled
HiLyte™ Fluor-labeled Secondary Antibodies
HiLyte™ Fluor-labeled Streptavidins
HiLyte™ Fluor dyes
AnaTag™ Protein Labeling Kits

Request the new HiLyte™ Fluor brochure - to view please click here