pleased to introduce the newest member of the HiLyte™ Fluor family - HiLyte™ Fluor
594 (Ex/Em=593/616 nm). It is a novel bright and red fluorescent probe.
dyes are a series of superior fluorescent labeling dyes that span the full visible
spectrum. These dyes have been optimized to yield maximum brightness when conjugated
HiLyte™ Fluor 594 has spectral characteristics similar to those of Texas Red®
(a trade mark of Invitrogen for sulforhodamine 101 sulfonyl chloride), with
excitation and emission wavelength at 593/615 nm in PBS buffer (pH 7.4), and
596/617 nm when conjugated to macromolecules (see spectra below). The labeling
performance and stability properties of HiLyte™ Fluor 594 are better than those
of Texas Red®. It has a higher extinction coefficient (80,000 M-1cm-1)
and a lower correction factor (0.17) compared to Alexa Fluor™ 594 (Invitrogen).
The fluorescence quantum yield of HiLyte™ Fluor 594 is 0.9 in aqueous solution.
HiLyte™ Fluor 594 conjugated streptavidin provides high fluorescence intensity
and low background as validated in immunofluorescence staining of mammalian
cells. Bioconjugates conjugated to HiLyte™ Fluor 594 exhibit little spectral
overlap with green-fluorescent conjugates, and can be efficiently excited by
568 nm line of Ar-Kr laser and by the 594 nm line of orange He-Ne laser.
Fluor-labeled Secondary Antibodies
pleased to highlight the newly developed HiLyte™ Fluor 594 (in a
variety of reactive forms) and HiLyte™ Fluor 594 conjugated proteins.
These fluorescent reagents are of the highest quality, cost-effective and available for quick delivery.