HiLyte™ Fluor 594 - Red and Bright


AnaSpec is pleased to introduce the newest member of the HiLyte™ Fluor family - HiLyte™ Fluor 594 (Ex/Em=593/616 nm). It is a novel bright and red fluorescent probe.

HiLyte™ Fluor dyes are a series of superior fluorescent labeling dyes that span the full visible spectrum. These dyes have been optimized to yield maximum brightness when conjugated with macromolecules.

Available in a variety of reactive forms, such as amine-reactive, thiol-reactive and amine-containing derivatives - allowing easy conjugation to biopolymers. HiLyte™ Fluor 594 has spectral characteristics similar to those of Texas Red® (a trade mark of Invitrogen for sulforhodamine 101 sulfonyl chloride), with excitation and emission wavelength at 593/615 nm in PBS buffer (pH 7.4), and 596/617 nm when conjugated to macromolecules (see spectra below). The labeling performance and stability properties of HiLyte™ Fluor 594 are better than those of Texas Red®. It has a higher extinction coefficient (80,000 M-1cm-1) and a lower correction factor (0.17) compared to Alexa Fluor™ 594 (Invitrogen). The fluorescence quantum yield of HiLyte™ Fluor 594 is 0.9 in aqueous solution. HiLyte™ Fluor 594 conjugated streptavidin provides high fluorescence intensity and low background as validated in immunofluorescence staining of mammalian cells. Bioconjugates conjugated to HiLyte™ Fluor 594 exhibit little spectral overlap with green-fluorescent conjugates, and can be efficiently excited by 568 nm line of Ar-Kr laser and by the 594 nm line of orange He-Ne laser.



HiLyte™ Fluor 594 conjugated proteins have been optimized in a specific fluorophore / protein labeling ratio to ensure a high fluorescent signal and uncompromised protein functions. The HiLyte™ Fluor 594-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications.

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Product Size Catalog #

HiLyte™ Fluor 594 acid, SE

1 mg

5 mg

81272-1

81272-5

HiLyte™ Fluor 594 acid

10 mg

81271

HiLyte™ Fluor 594 amine TFA salt

1 mg

81273

HiLyte™ Fluor 594 C2 maleimide

1 mg

81275

HiLyte™ Fluor 594 hydrazide

1 mg

81274


Product

Size

Catalog #

Goat anti-rabbit IgG (H+L), HiLyte™ Fluor 594 labeled

500 µg

28176-H594

Goat anti-rabbit IgG (H+L), highly cross-adsorbed, HiLyte™ Fluor 594 labeled

500 µg

61056-H594

Goat anti-mouse IgG (H+L), HiLyte™ Fluor 594 labeled

500 µg

28175-H594

Goat anti-mouse IgG (H+L), highly cross-adsorbed, HiLyte™ Fluor 594 labeled

500 µg

61057-H594

Rabbit anti-mouse IgG (H+L), HiLyte™ Fluor 594 labeled

500 µg

28164-H594

Rabbit anti-goat IgG (H+L), HiLyte™ Fluor 594 labeled

500 µg

28168-H594

Rabbit anti-chicken IgY (H+L), HiLyte™ Fluor 594 labeled

500 µg

29709-H594

Streptavidin, HiLyte™ Fluor 594 conjugated

1 mg

60672-H594

Anti-GST Tag HiLyte™ Fluor 594 labeled

100 µg

29531-H594

Anti-Actin (Smooth muscle specific) HiLyte™ Fluor 594-labeled NE

50 µg

29553-H594



Related Products:

HiLyte™ Fluor dyes

AnaTag™ Protein Labeling Kits

HiLyte™ Fluor-labeled Secondary Antibodies

HiLyte™ Fluor-labeled Streptavidins

AnaSpec is pleased to highlight the newly developed HiLyte™ Fluor 594 (in a variety of reactive forms) and HiLyte™ Fluor 594 conjugated proteins. These fluorescent reagents are of the highest quality, cost-effective and available for quick delivery.