HiLyte™ Fluor 594 - Red and Bright


AnaSpec is pleased to introduce the newest member of the HiLyte™ Fluor family - HiLyte™ Fluor 594 (Ex/Em=593/616 nm). It is a novel bright and red fluorescent probe.

HiLyte™ Fluor dyes are a series of superior fluorescent labeling dyes that span the full visible spectrum. These dyes have been optimized to yield maximum brightness when conjugated with macromolecules.

HiLyte™ Fluor 594 has spectral characteristics similar to those of Texas Red® (a trade mark of Invitrogen for sulforhodamine 101 sulfonyl chloride), with excitation and emission wavelength at 593/615 nm in PBS buffer (pH 7.4), and 596/617 nm when conjugated to macromolecules (see spectra below). The labeling performance and stability properties of HiLyte™ Fluor 594 are better than those of Texas Red®. It has a higher extinction coefficient (80,000 M-1cm-1) and a lower correction factor (0.17) compared to Alexa Fluor™ 594 (Invitrogen). The fluorescence quantum yield of HiLyte™ Fluor 594 is 0.9 in aqueous solution. HiLyte™ Fluor 594 conjugated streptavidin provides high fluorescence intensity and low background as validated in immunofluorescence staining of mammalian cells. Bioconjugates conjugated to HiLyte™ Fluor 594 exhibit little spectral overlap with green-fluorescent conjugates, and can be efficiently excited by 568 nm line of Ar-Kr laser and by the 594 nm line of orange He-Ne laser.



 

Product Size Catalog #

HiLyte™ Fluor 594 acid, SE

1 mg

5 mg

81272-1

81272-5

HiLyte™ Fluor 594 acid

10 mg

81271

HiLyte™ Fluor 594 hydrazide

1 mg

81274




Related Products:

HiLyte™ Fluor dyes

AnaTag™ Protein Labeling Kits

HiLyte™ Fluor-labeled Secondary Antibodies

HiLyte™ Fluor-labeled Streptavidins

AnaSpec is pleased to highlight the newly developed HiLyte™ Fluor 594 (in a variety of reactive forms) and HiLyte™ Fluor 594 conjugated proteins. These fluorescent reagents are of the highest quality, cost-effective and available for quick delivery.