pleased to announce the release of the industry's first longest wavelength FRET
based calpain assay, the SensoLyte® 520 Calpain Activity Assay Kit. Optimized for detecting calpain activity, this kit contains
a novel internally quenched 5-FAM/QXL™ 520 FRET substrate. Cleavage
of this FRET substrate generates two separate fragments. The subsequent release
of 5-FAM fluorescence can be monitored at excitation/emission= 490/520 nm. Increase
in fluorescence is proportional to the calpain activity. The assay can detect
both calpain 1 (m) and 2 (m) activities and is ideal
for kinetic study of these enzymes. The long wavelength fluorescence of 5-FAM
is less interfered by the autofluorescence of components in biological samples
and test compounds.
Calpains are a family of intracellular cysteine proteases, which
consists of at least 15 ubiquitous and tissue-specific isoforms.1
The best-characterized calpains are the isoforms µ- and m-calpain,
which are also known as calpain 1 and calpain 2, respectively. Calpains
are calcium-dependent and function by modulating the biological activities of
many proteins through selective cleavage.2 Studies have demonstrated
that calpains are implicated in a variety of calcium-regulated
cellular processes such as signal transduction, cell proliferation,
differentiation, cell cycle progression, apoptosis, and platelet activation.
Deregulation of calpains activities has been implicated in various
pathological phenomena such as atherosclerosis, Alzheimer's
disease, diabetes, and cancer.3, 4 Calpains represent potential
therapeutic targets for drug development.5, 6
An AMC based
calpain assay, the SensoLyte®
AMC Calpain Assay, is also available from AnaSpec.
Modified Beta-Amyloid Peptides – Accelerated Amyloidogeneity
Peptides - The World's Most Comprehensive Collection
of β-Amyloid GO™ Peptides
(1-42) Sampler Kit
human protein, HiLyte™ Fluor 488 labeled (Ex/Em = 490/520 nm)
1. Goll, DE. et al. Physiol. Rev. 83,
2. Suzuki K, et al. Diabetes.
53 Suppl 1, S12 (2004).
3. Zatz M, et al: N Engl J Med. 352,
4. Branca D. Biochem
Biophys Res Commun. 322, 1098 (2004).
NO. Curr Pharm Des. 12, 615 (2006).
6. Saez ME. et al. Drug Discovery
Today 11, 917 (2006).