AnaSpec is pleased to announce the release of the industry's first and
longest wavelength FRET based Cathepsin K assay, the SensoLyte®
520 Cathepsin K Assay Kit. Optimized for detecting Cathepsin
K activity, this kit contains a novel internally quenched
5-FAM/QXL™ 520 FRET substrate. Upon cleavage of this FRET
substrate, two separate fragments are generated resulting in the release
of 5-FAM fluorescence. Fluorescence can be monitored at Ex/Em= 490/520 nm. Increase
in fluorescence is proportional to the Cathepsin K activity. The SensoLyte®
520 Cathepsin K Assay Kit is AnaSpec's most specific assay kit in its catalog.
samples containing mixed cathepsins, AnaSpec offers the SensoLyte® Rh110 Cathepsin K Assay Kit. Spectrally
similar to the SensoLyte 520 assay kit, the Rh110 substrate is cleaved by Cathepsin
L (if present in samples) at a faster rate than the FRET substrate provided
in the 520 kit.
include long wavelength fluorescent dyes, 5-FAM and Rh110, that are subject
to less interference by the autofluorescence of components in biological samples
and test compounds.
K is a cysteine protease that plays a role in the degradation of protein components
of the bone matrix during bone resorption.1 Produced by bone resorbing
macrophages and synovial fibroblasts, Cathepsin K cleaves proteins such as collagen
type I, collagen type II and osteonectin.2
It has potential as a drug target in autoimmune diseases and osteoporosis.3,
SensoLyte® 520 Cathepsin S Activity Assay Kit (Ex/Em =
490 nm/520 nm)
SensoLyte® 440 Cathepsin S Activity Assay Kit (Ex/Em =
354 nm/442 nm)
390 Cathepsin D Activity Assay Kit (Ex/Em = 330 nm/390 nm)
Substrates, Inhibitors & Peptide
of β-Amyloid GO™ Peptides
(1-42) Sampler Kit
human protein, HiLyte™ Fluor 488 labeled (Ex/Em = 490/520 nm)
Motyckova, G. et al. PNAS. 98 ,
Hou, WS. et al. Am. J. Pathol. 159, 2167 (2001).
M. et al. Arthritis Res Ther. 7, 65 (2005).
M. et al. Science 319, 624 (2008).