AnaSpec, the maker of the industry's first and only colorimetric anti-MOG assay
kits, is pleased to announce the release of two multiple sclerosis (MS)/experimental
autoimmune encephalomyelitis (EAE) related ELISA assays kits, the SensoLyte®
Kit and the SensoLyte®
Kit. These kits, precoated either with PLP (139-151) or PLP
(178-191) peptide are optimized to detect mouse anti- PLP (139-151)
or anti-PLP (178-191) IgG, respectively. The amount of IgG antibodies
in mouse serum or cerebrospinal fluid is quantified using ELISA. Ample materials
and reagents are provided to perform 80 assays in a 96-well plate format.
• Convenient Format
— Pre-coated and pre-blocked 96-well strip plate
— Ready-to-use substrate solution and other assay
— 2-3 hours assay time at room temperature
• Minimal Sample Size
— Requires only 0.5-1 μl
of serum or cerebrospinal fluid to perform assay
• High Sensitivity
— Detects as low as 100 pg of anti-PLP (139-151)
or anti-PLP (178-191) IgG
— No cross-reactivity between two kits
• Broad Dynamic Range
— 8-500 ng antibody/ml serum (depending on colorimetric
Proteolipid protein (PLP) is the most abundant protein in the central nervous
system (CNS) myelin sheath and is highly conserved among species.1-2
PLP has a 50% hydrophobic amino acids content and a molecular weight of 30 kDa.1
Mouse PLP (139-151) and PLP (178-191) are able
to induce autoantibody production and relapsing-remitting neurological disease
causing extensive plaque-like demyelination.1-4 Autoantibody response
to mouse PLP (139-151) and PLP (178-191) has
been observed in induced experimental autoimmune encephalomyelitis (EAE) in
B10.PL, C57BL/6, BALB/c, SJL, F1, and SWR mice strains.3-4 However,
the exact pathological role and action of anti-mouse PLP (139-151)
and anti-PLP (178-191) autoantibody is not well understood.1-4
Figure 1. The standard curve for anti-mouse PLP (139-151) on the left and anti-mouse PLP (178-191)
on the right.
Proteins, Peptides, Antibodies & Assay Kits
View a poster on Suppressive Role of CD72 in Experimental Autoimmune Encephalomyelitis
1 Tzakos, AG. et al. Curr.
Med. Chem.12, 1569 (2005).
2 Miller, D. et al. Curr.
Protocols Immunol .15.1.1.(2007).
3 Tuohy, V. et al. J.
Neuroimmunol.. 39,67 (2003).
4 Kuchroo, V. et al. Pathobio.
59, 305 (1991).