Human α-Synuclein (1-140) – Biotin-Labeled


AnaSpec, the provider of the world’s largest collection of b-amyloid peptides is pleased to announce the recent release of biotin-labeled human a-synuclein. For use in neurodegenerative research, specifically for Parkinson’s disease (PD) research, this 140-amino acid long recombinant protein (GenBank Accession # NP_000336) was expressed and purified from E. coli and subsequently labeled with biotin. α-Synuclein (1-140) labeled with AnaSpec’s proprietary HiLyte™ Fluor 488 dye and non-labeled α-Synuclein (1-140) are also available. These proteins can be used in applications such as ELISA, western blots and microscopy imaging.

α- Synuclein (1-140) biotin labeled (Cat# 55581) α-Synuclein (1-140) HiLyte™ Fluor 488 labeled (Cat# 55457) α-Synuclein (1-140) (Cat# 55456)
Amount 200 ug 200 ug 500 ug
Purity (SDS-PAGE & MS)
>95% >95% >95%
DOS* 6.55 2.23 NA
Ex/Em (nm) NA 503/525 NA

Parkinson's disease is predominantly a movement disorder resulting from degeneration of dopaminergic neurons in the substantia nigra. The cause of the disease is unknown, but substantial evidence suggests that the aggregation of a-synuclein is a critical step in the etiology of Parkinson's disease. a-Synuclein is an abundant brain protein of 140 residues that is present in high concentrations at presynaptic terminals and is found in both soluble and membrane-associated fractions of the brain. Several possible functions have been suggested for α-synuclein, and it also appears to be involved in vesicle release and trafficking. In vitro incubation in the presence of a specific amount of salt (i.e. 0.1M NaCl) with agitation shows α-synuclein forming fibrilous structure.



Figure 1. SDS-PAGE analysis of biotinylated human
a-synuclein (cat# 55581) and HiLyte™ Fluor 488-labeled human a-synuclein (cat# 55457) showed a single band at ~17 kDa position. Lane A: unlabeled; Lane B: HiLyte™ Fluor 488-labeled human a-synuclein; Lane C: biotin labeled human a-synuclein.

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References
  1. Trojanowski, JQ. & VM. Lee, Ann. N. Y. Acad. Sci. 991, 107 (2003).
  2. Masliah, E. et al. Science 287, 1265 (2000).
  3. Van Der, PH, et al. J. Neurosci. 20, 6021 (2000).
  4. Feany, MB. & WW. Bender, Nature 404, 394 (2000).
  5. Weinreb, PH., et al. Biochemistry 35, 13709 (1996).