AnaSpec, Eurogentec Group, is pleased to announce the release of the industry's
first fluorimetric kits for Cathepsin G, the SensoLyte®
520 Cathepsin G Assay Kit and SensoLyte®
Rh110 Cathepsin G Assay Kit. These long-wavelength, homogeneous assays
are ideal for the screening of enzyme inhibitors and activators or for continuous
assay of Cathepsin G activity. The SensoLyte® 520 Cathepsin G Assay
Kit contains a FRET-based (HiLyte FluorTM 488/QXLTM 520) substrate and the SensoLyte®
Rh110 Cathepsin G Assay Kit, a fluorogenic substrate labeled with Rh110. These 2 substrates
have excitation/emission at the green wavelengths of 490/520 nm. The longer
wavelength spectra and higher extinction coefficient of HiLyte FluorTM 488 and
Rh110 provide greater sensitivity and less interference from other reaction
Cathepsin G is a neutrophil serine protease,
stored in cytoplasmic granules and secreted into the extra- and pericellular
space upon neutrophil activation.1 Cathepsin G plays important role
in bacterial defense. It acts in combination with reactive oxygen species to
help degrade engulfed microorganisms inside phagolysosomes.2,3 Besides
bactericidal effect, Cathepsin G regulates the inflammatory response by specifically
altering the function of cytokines and chemokines.4 Because of its
role in host defense, Cathepsin G is of interest as a potential therapeutic
Figure 1. Inhibition of Cathepsin G activity by Cathepsin G
inhibitor as measured with SensoLyte® 520 Cathepsin G activity
Assay Kit (Cat# 72185).
Figure 2. Cathepsin titration with SensoLyte® Rh110 Cathepsin
G activity Assay Kit (Cat# 72186). Kit can detect 0.6 ng of Cathepsin G after
1 hour incubation of enzyme with substrate.
1. Egesten, A. et al. Blood
83, 2985 (1994).
2. Korkmaz, B. et al. Biochimie.
90, 227 (2008).
3. Sieńczyk M. et al. Bioorg
Med Chem. 19,8863 (2008).
4. Pham, CT. Nat Rev Immunol.
6, 541 (2006).
5. Korkmaz, B. et al. Pharmacol
Rev. 62, 726 (2010).
6. Lesner, A. et al. Anal Biochem.
375, 306 (2008).
7. Greco, MN. et al. J Am Chem
Soc. 124, 3810 (2002).