SensoLyte® Cathepsin G Kits – Industry's 1st Fluorimetric Assays


AnaSpec, Eurogentec Group, is pleased to announce the release of the industry's first fluorimetric kits for Cathepsin G, the SensoLyte® 520 Cathepsin G Assay Kit and SensoLyte® Rh110 Cathepsin G Assay Kit. These long-wavelength, homogeneous assays are ideal for the screening of enzyme inhibitors and activators or for continuous assay of Cathepsin G activity. The SensoLyte® 520 Cathepsin G Assay Kit contains a FRET-based (HiLyte™ Fluor 488/QXL™ 520) substrate and the SensoLyte® Rh110 Cathepsin G Assay Kit, a fluorogenic substrate labeled with Rh110. These 2 substrates have excitation/emission at the green wavelengths of 490/520 nm. The longer wavelength spectra and higher extinction coefficient of HiLyte™ Fluor 488 and Rh110 provide greater sensitivity and less interference from other reaction components.

Cathepsin G is a neutrophil serine protease, stored in cytoplasmic granules and secreted into the extra- and pericellular space upon neutrophil activation.1 Cathepsin G plays important role in bacterial defense. It acts in combination with reactive oxygen species to help degrade engulfed microorganisms inside phagolysosomes.2,3 Besides bactericidal effect, Cathepsin G regulates the inflammatory response by specifically altering the function of cytokines and chemokines.4 Because of its role in host defense, Cathepsin G is of interest as a potential therapeutic target.5


Figure 1. Inhibition of Cathepsin G activity by Cathepsin G inhibitor as measured with SensoLyte® 520 Cathepsin G activity Assay Kit (Cat# 72185).


Figure 2. Cathepsin titration with SensoLyte® Rh110 Cathepsin G activity Assay Kit (Cat# 72186). Kit can detect 0.6 ng of Cathepsin G after 1 hour incubation of enzyme with substrate.

Product

Catalog #

SensoLyte® 520 Cathepsin G Assay Kit *Fluorimetric*

72185

SensoLyte® Rh110 Cathepsin G Assay Kit *Fluorimetric*

72186

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Cathepsin Related Peptide Substrates

References

1. Egesten, A. et al. Blood 83, 2985 (1994).
2. Korkmaz, B. et al. Biochimie. 90, 227 (2008).
3. Sieńczyk M. et al. Bioorg Med Chem. 19,8863 (2008).
4. Pham, CT. Nat Rev Immunol. 6, 541 (2006).
5. Korkmaz, B. et al. Pharmacol Rev. 62, 726 (2010).
6. Lesner, A. et al. Anal Biochem. 375, 306 (2008).
7. Greco, MN. et al. J Am Chem Soc. 124, 3810 (2002).