AnaSpec, Eurogentec Group, is pleased to be the first in the industry
to establish a new product line of zebrafish specific (Z-Fish™) antibodies
to support the increasing needs of researchers.
Our expanding list of Z-Fish™ antibodies includes targets used
in transcriptional regulation, signal transduction, apoptosis and autophagy,
cell cycle control, cytoskeleton, glucose and energy metabolism, and immunity
research. Antibodies were verified in ELISA. Many were verified in western blots
using zebrafish lysates.
As well, these Z-Fish™ antibodies are predicted to work in IHC, many
of which have good IHC results. Antibodies are supplied in 150 ul size, this
amount is enough for 15 mini gels.
In collaboration with the University
of Utah, Salt Lake City, Utah, AnaSpec's Z-Fish™ polyclonal anti-lysozyme was
found to completely overlap with mpo:GFP labeled neutrophils. This indicates
that lysozyme is a neutrophil marker in zebrafish (Figure 1). Lysozyme C (lyz), a cationic
glycoside hydrolase, breaks down N-acetyl muramic b-1,4 N-acetyl glucosamine linkages in the bacterial cell
wall.1-2 In zebrafish, this enzyme is expressed exclusively in myeloid
cells,2 and is used for hematopoietic developmental studies in zebrafish.
This antibody was raised using a synthetic peptide derived from
the intermediate region of zebrafish lysozyme protein (GenBank accession # NP_631919.1).
Host: rabbit polyclonal; Species reactivity: zebrafish;
Figure 1. Whole-mount zebrafish IHC of a 3 dpf larvae
probed with Z-Fish™ anti-lysozyme (IN), (cat# 55633) at 1:25-1:200 dilution.
Fixation conditions were 4% PFA with 0.1% Tween 20 overnight at 4 degrees. Anti-Lysozyme
antibody stains neutrophils in red. Anti-Lysozyme label
overlaps almost completely with the mpo:GFP labeled neutrophils, indicating that
lysozyme is a neutrophil marker (image courtesy of Dr. Michael Redd, Univ. of Utah).
- Gordon, S. et al. J Exp Med 139(5), 1228 (1974).
- Kitaguchi, T. et al. Mech Dev 126, 314 (2009).
for the following targets:
and Cell Matrix Adhesion
Matrix/ MMP/ TIMP
/ Growth Factor
Binding and Protein Folding
Transduction and Others