Custom Assay Development Service

AnaSpec, EGT Group, provider of the long wavelength, ultra-sensitive SensoLyte assay kits, is pleased to offer custom service for the development of assays. Our long wavelength SensoLyte protease assays, many of which utilize the 5-FAM/QXL 520 and HiLyte Fluor 488/QXL 520 FRET pairs, have revolutionized the industry, setting the gold standard in fluorimetric assay sensitivity (Figure 1). Our expertise in peptide chemistry, combined with extensive experience in the syntheses of fluorescent dyes and quenchers, have resulted in the development of ultra-sensitive, convenient, time-saving, cost-effective assays. Researchers from the diagnostic, biotechnology, pharmaceutical and academic sectors have reported the use of our assays in numerous publications in high impact journals.

Committed to the highest levels of performance, our custom assay development team will work with you to develop assays that will meet or exceed your specific manufacturing and research priorities. Complete confidentiality of all orders is assured.

Design of optimized peptide substrates
FRET, fluorimetric or luminometric technology
Novel long wavelength FRET pairs, such as HiLyte Fluor 488/QXL 520
& 5-FAM/QXL 520
Homogeneous assay
Ideal for HTS (96 or 384-well format)
Assay validation with purified enzyme and/or known inhibitor
Validation with known inhibitor
Assay parameters information included (sensitivity, linear range)
Detailed protocol provided

Figure 1. The 5-FAM/QXL 520 renin FRET substrate is 40X more sensitive than the traditional EDANS/DABCYL FRET substrate and 10X more sensitive than an Amp/Dnp FRET substrate. It can detect 0.8 ng/ml of renin.

Custom Inhibitor Screening Service

In addition to custom assay development service, AnaSpec also provides custom inhibitor screening service for proteases such as HCV/HIV proteases; renin; MMPs; cathepsin B, D, G, K, S and others.

Click here to request a quotation.

Select literature citations:

SensoLyte520 Renin Assay Kits

Sbroggio, M. et al. Cardiovasc Res. doi: 10.1093/cvr/cvr103 (2011).
Feliers, D. et al. Mol. Cell. Endocrinol. 314, 136(2010).
Iwai, M. et al. J. Hypertens. 28, 147(2010).
Du, D. et al. Mol. Biotech. 43, 1559 (2009).
Schmiedt, C. et al. Am. J. Vet. Res. 70, 1315 (2009).
ivn, M. et al. Am. J. Hum. Genet. 85, 204 (2009).

SensoLyte 520 MMP Assay Kits

Mak, I. et al. Biochem. Res. Int. doi:10.1155/2011/164197 (2011).
Leong, D. et al. FASEB J. 25, 182 (2011).
Williams, J. et al. AJP Renal Physiol. doi: 10.1152/ajprenal.00262.2010 (2011).
Slanina, H. et al. J. Microbiol. Met. 84, 101 (2010).
Spiga, MG. And T. Borras Invest. Ophthal. Vis. Sc. doi:10.1167/iovs.09-4918 (2010).
Corriveau, MP. et al. J. Pathol. 217, 534 (2009).
Gueders, MM. et al. Biochem. Pharma. 75, 514 (2008).
Adiseshaiah, P. et al. J. Cell. Physiol. 216, 405 (2008).
Johnson, SL. et al. Bioorganic Chem. 35, 306 (2007).
Yi, F. et al. Kidney Int. 70, 88 (2006).

SensoLyte 520 TACE Assay Kits

Zhao, XQ. et al. J. Lipid Res. doi: 10.1194/jlr.M015156 (2011).
Hassemer, EL. et al. Genetics doi:10.1534/genetics.109.113167 (2010).
Hurst, LA. et al. J Neuroimmunol 210, 108 (2009).
Walker, EJ. and GA. Rosenberg Exp Neuro 216, 122(2009).
Cardellini, M. et al. Diabetes 58, 2396 (2009).