SensoLyte® Anti-α-Synuclein assay kits

AnaSpec, EGT Group is pleased to announce the release of an ultrasensitive ELISA assay for quantitatingα-synuclein. The SensoLyte® α-Synuclein Quantitative ELISA Kit (Human/Mouse/Rat) provides a convenient and quantitative assay for determining α-Synuclein amount in cell and tissue lysate as well as in body fluids. Compared to other anti-α-Synuclein ELISA kits on the market, this one-step, mix-and-read assay takes less time to run. HRP conjugated detection antibody is added simultaneously with the samples and standards during the assay, eliminating extra incubation and washing steps.


Catalog #

SensoLyte® Anti-α-Synuclein (Human) ELISA Kit


One Step Convenient Format
* Pre-coated and pre-blocked 96-well strip plate
* Ready-to-use substrate solution and other assay components
* One step assay (samples and detection antibody are added simultaneously)
* 1 hour assay time at room temperature (excluding incubation time)

§ Minimal Sample Size
* Requires only 10-20 μl of cell and tissue lysate or body fluids

§ High Sensitivity
* Detects 5 pg/ml of α-Synuclein (calculated as three standard deviations from the blank)

§ Broad Dynamic Range
* 8-500 pg α-Synuclein/ml in the assayed sample

Figure 1. An example of the α-Synuclein ELISA standard curve

α-Synuclein is a major component of Lewy bodies found in affected neurons of patients with Parkinson's disease.1-4 This protein has a mass of 14.5 kDa (140 amino acids long) and consists of a conserved degenerative amino-terminal domain and an acidic carboxyl-terminal that has a higher sequence divergence.1-4 α-Synuclein is predominantly expressed in brain, specifically in cerebellum, thalamus, neocortex, hippocampus, and striatum regions.1-2 Other tissues also express α-Synuclein but at very low levels.1-4 The physiological role of α-Synuclein is not well understood. However, the presence of imperfect KTKEGV lipid interacting repeats suggests that it may be involved in synaptic vesicle homeostasis.3

Related Products

Table 1. A listing of Synuclein recombinant proteins



Catalog #

Recombinant human α - Synuclein (1 - 140)

500 µg

1000 µg



Recombinant human α - Synuclein (1 - 140), biotin labeled

200 µg


Recombinant human α - Synuclein (1 - 140), HiLyte™ Fluor 488 labeled

200 µg


Recombinant Human β - Synuclein (1 - 134)

100 µg

500 µg

1000 µg




Table 1. A listing of Synuclein antibodies.



Catalog #

Anti-α-Synuclein (pSer 87)

25 µg

50 µg



Anti-α-Synuclein (paired 87)

50 µg


Anti-Synuclein (pan)(IN)

50 µg



Prusiner, B. S. (2015). Evidence for α-synuclein prions causing multiple system atrophy in humans with parkinsonism pnas 112 doi: 10.1073/pnas.1514475112.

Woerman, A. L. (2015). Propagation of prions causing synucleinopathies in cultured cells. PNAS 112 doi: 10.1073/pnas.1513426112.

Al-Nimer, MS. et al. (2014). Saliva α-synuclein and a high extinction coefficient protein: A novel approach in assessment biomarkers of Parkinson's disease. North Am J Med Sci 6, 633. doi: 10.4103/1947-2714.147980.

Maia, LF. et al. (2013). Changes in amyloid-β and tau in the cerebrospinal fluid of transgenic mice overexpressing amyloid precursor protein. Sci Transl Med 5, 194re2.

Smith, LM. et al. (2012). α-Synuclein and anti-α-synuclein antibodies in Parkinson’s disease, atypical Parkinson syndromes, REM sleep behavior disorder, and healthy controls. PloS One 7, e52285.


  1. Rivers, R. et al. Protein Science 17, 887 (2008).
  2. Bruening, W. et al. Cancer 88, 2154 (2000).
  3. George M. J. Genome Biology3, 1 (2001).
  4. Latawiec, D. et al. PloS ONE 5, 1 (2010).