[Me2s] & [Me2a]-Histone Peptides


AnaSpec, EGT Group offers one of the world's largest selections of Histone GO (catalog) peptides. Our peptides include sequences containing modified amino acids such as methylated (Me1, Me2 and Me3) Lysine; phosphorylated Threonine (pThr) or Serine (pSer); deiminated Arginine (Cit). We also offer symmetric and asymmetric dimethylated Arginine (Me2s and Me2a) in addition to monomethylated Arginine (Me1).

Methylation of arginine residues are mediated by members of the protein arginine methyltransferases (PRMTs) family. PRMT type I adds asymmetrically dimethyl groups to arginine (ADMA), while type II add symmetrically dimethyl groups (SDMA). Type III adds monomethyl group to arginine.1 Type 1 PRMT, Coactivator-associated arginine methyltransferase 1 (CARM1), also known as PRMT4, methylates Histone 3 at R2, R17 and R26. CARM1 methylation of H3R17 activates transcription.2-4 PRMT5, which catalyzes H3R8 and H4R3 symmetric dimethylation leads to repression of gene expression, while PRMT1 asymmetric dimethylation of H4R3 is associated with gene activation.4, 5

AnaSpec, EGT Group's dimethylated arginines peptide sequences for H3 and H4 are listed in Tables 1 and 2, respectively. Where both versions are available for the same sequence, the symmetrical version is listed on top, with the asymmetrical equivalent below it. Fmoc symmetrical and asymmetrical dimethylated arginines are listed in Table 3.

Large collection of histone peptides

Peptides include sequences that have acetylated, methylated, phosphorylated and citrullinated amino acids

Peptides manufactured in California, USA

Table 1. A listing of Histone H3 sequences containing symmetrical and asymmetrical dimethylated arginines.

Product

Size

Catalog #

[Arg(Me2s)8]-Histone H3 (1-21)-K(biotin), H3R8(Me2s)
ARTKQTA-R(Me2s)-KSTGGKAPRKQLA-K(Biotin)

1 mg

AS-65351-1

[Arg(Me2a)8]-Histone H3(1-21)-K(Biotin), H3R8(Me2a)
ARTKQTA-R(Me2a)-KSTGGKAPRKQLA-K(Biotin)-NH2

1 mg

AS-64972-1

[Arg(Me2a)26]-Histone H3 (15-36)-GGK(Biotin)
APRKQLATKAA-R(Me2a)-KSAPATGGVKGG-K(Biotin)

1 mg

AS-65261-1

Table 2. A listing of Histone H4 sequences containing symmetrical and asymmetrical dimethylated arginines.

Product

Size

Catalog #

[Arg(Me2a)3]-Histone H4(1-21)-GGK(Biotin), H4R3(Me2a)
Ac-SG-R(Me2a)-GKGGKGLGKGGAKRHRKVGG-K(Biotin)

1 mg

AS-64976-1

[Arg(Me2s)3]-Histone H4 (1-23)-GGK(Biotin)
SG-R(Me2s)-GKGGKGLGKGGAKRHRKVLRGG-K(Biotin)

1 mg

AS-65276-1

[Arg(Me2a)3]-Histone H4 (1-23)-GGK(Biotin)
SG-R(Me2a)-GKGGKGLGKGGAKRHRKVLRGG-K(Biotin)

1 mg

AS-65278-1

Table 3. A listing of Fmoc symmetrical and asymmetrical dimethylated arginines.

Product

Size

Catalog #

Fmoc-Arg(Me)2-OH (symmetrical)
Fmoc-Nω,ω-dimethyl-L-arginine (symmetrical)

250 mg

1 g

AS-26217-F025

AS-26217-F1

Fmoc-Arg(Me)2-OH (asymmetrical)
Fmoc-Nω,ω-dimethyl-L-arginine
(asymmetrical)

250 mg

1 g

AS-26218-F025

AS-26218-F1

Reference:

1. Bedford MT, et al. Mol Cell 18, 263 (2005).
2. Chen, D. et al. Science 284, 2174 (1999).
3. Ma, H. et al. Curr Biol 11, 1981 (2001).
4. Schurter, BT. et al. Biochem 40, 5747 (2001).
5. Pal, S. et al. Mol Cell Biol 24, 9630 (2004).