MOG Proteins

Customers' Feedback on AnaSpec's MOG proteins:

Feedback #1: Your Recombinant Human MOG (1-125), Cat# 55158 is excellent in quality; technical support is also excellent. The datasheet is very well-written and delivery was excellent.

Feedback #2: Your Recombinant Rat MOG (1-125), Cat# 55152 is excellent in quality.

Our MOG proteins, with sequences corresponding to human, mouse and rat extracellular domain, together with a 6x His tag were expressed in E. coli and purified from urea denatured bacterial lysate. The molecular weight found in all 3 recombinant proteins was 14.23 kDa. All of these proteins have been shown to induce EAE.

Myelin Oligodendrocyte Glycoprotein (MOG), a 26- to 28-kDa protein, is a member of the immunoglobulin superfamily. It is expressed exclusively in the central nervous system (CNS), specifically on the surface of myelinating oligodendrocytes and external lamellae of myelin sheath. Although MOG protein constitutes only 0.01-0.05% of the CNS myelin proteins, it was demonstrated that MOG protein is a crucial autoantigen for multiple sclerosis in humans and experimental autoimmune encephalomyelitis (EAE) in rodents and monkeys (1-6).

Recombinant MOG Protein

Size

Catalog #

Recombinant human MOG

100 g

500 g

1000 g

AS-55158-100

AS-55158-500

AS-55158-1000

Recombinant mouse MOG

100 g

500 g

1000 g

AS-55150-100

AS-55150-500

AS-55150-1000

Recombinant rat MOG

100 g

500 g

1000 g

AS-55152-100

AS-55152-500

AS-55152-1000G



Figure 1. Purified recombinant MOGs (rMOG) was loaded onto 10-20% Tris-HCl SDS-PAGE (10 μg/well) and resolved at 200V for 60 minutes. Protein markers and the purified rMOG (14.2 kDa) are indicated. CL= Crude Cell Lysate, FT= Flow Through, and P = Purified rMOG.


Figure 2. Average EAE score of five female Dark Agouti rats (8 weeks old) injected with human MOG (1-125)

Related Products:
MOG Peptides
Anti-MOG Antibodies
SensoLyte Anti-MOG IgG ELISA Assay Kits
MBP Peptides
PLP Peptides
Anti-MBPs

References:

1. Linares, D. et al. Protein Expression and Purif. 34, 249 (2004).
2. Bettadapura, J. et al. J. Neurochem. 70, 1593 (1998).
3. Oliver, AR. et al. J. Immunol. 171, 462 (2003).
4. Von Budingen, H-C. et al. J. Clin. Immunol. 21, 155 (2001).
5. Lyons, J-A. et al. Eur. J. Immunol. 29, 3432 (1999).
6. Von Budingen, H-C. et al. Eur. J. Immunol. 34, 2072 (2003).