Customers' Feedback on AnaSpec's MOG proteins:
Feedback #1: Your
Recombinant Human MOG (1-125), Cat# 55158 is excellent in quality; technical
support is also excellent. The datasheet is very well-written and delivery
Feedback #2: Your Recombinant Rat MOG (1-125), Cat# 55152 is excellent in quality.
Our MOG proteins, with sequences corresponding to human, mouse
and rat extracellular domain, together with a 6x His tag were expressed in E.
coli and purified from urea denatured bacterial lysate. The molecular weight
found in all 3 recombinant proteins was 14.23 kDa. All of these proteins have
been shown to induce EAE.
Myelin Oligodendrocyte Glycoprotein (MOG), a 26- to 28-kDa protein,
is a member of the immunoglobulin superfamily. It is expressed exclusively in
the central nervous system (CNS), specifically on the surface of myelinating
oligodendrocytes and external lamellae of myelin sheath. Although MOG protein
constitutes only 0.01-0.05% of the CNS myelin proteins, it was demonstrated
that MOG protein is a crucial autoantigen for multiple sclerosis in humans and
experimental autoimmune encephalomyelitis (EAE) in rodents and monkeys (1-6).
Figure 1. Purified recombinant MOGs (rMOG) was loaded onto 10-20%
Tris-HCl SDS-PAGE (10 μg/well) and resolved at 200V for 60 minutes. Protein
markers and the purified rMOG (14.2 kDa) are indicated. CL= Crude Cell Lysate,
FT= Flow Through, and P = Purified rMOG.
Figure 2. Average EAE score of five female Dark Agouti rats (8 weeks old) injected
with human MOG (1-125)
SensoLyte® Anti-MOG IgG
ELISA Assay Kits
1. Linares, D. et al. Protein Expression and Purif. 34, 249 (2004).
2. Bettadapura, J. et al. J. Neurochem. 70, 1593 (1998).
3. Oliver, AR. et al. J. Immunol. 171, 462 (2003).
4. Von Budingen, H-C. et al. J. Clin. Immunol. 21, 155 (2001).
5. Lyons, J-A. et al. Eur. J. Immunol. 29, 3432 (1999).
6. Von Budingen, H-C. et al. Eur. J. Immunol. 34, 2072 (2003).