AnaSpec, EGT Group, the provider of the ultra-sensitive SensoLyte®
520 Human Renin Assay Kit also provides renin kits specific for mouse and
rat - the SensoLyte®
520 Mouse Renin Assay Kit and SensoLyte®
520 Rat Renin Assay Kit. Using our proprietary 5-FAM/QXL™ 520 fluorescence
resonance energy transfer (FRET) peptide, all three assays are ideal for screening
of renin inhibitors and for continuous assay of renin activity, especially for
high throughput screening (HTS) purposes. In the intact FRET peptide, the fluorescence
of 5-FAM is quenched by QXL™ 520. However, upon cleavage into two separate fragments
by rat renin, the fluorescence of 5-FAM is recovered, and can be monitored at
excitation/emission = 490/520 nm. With a high fluorescence quantum yield and
long emission wavelength, the signal of 5-FAM can be detected with less interference
from the autofluorescence of cell components and test compounds. The assays
are performed in a convenient 96-well microplate format and can be adjusted
to 384-well microplate format.
• Ultra-sensitive assays
• FRET based substrates
• Long wavelength
• Minimal autofluorescence
• Renin assays kits specific for human, mouse & rat
The renin–angiotensin system (RAS) plays a central role in the
regulation of blood pressure and electrolyte homoeostasis.1 At the
first and rate-limiting step of the RAS cascade, renin, a highly specific aspartyl
protease, cleaves angiotensinogen, produced in the liver, to yield angiotensin
I, which is further converted into angiotensin II by ACE (Angiotensin Converting
Enzyme). Angiotensin II constricts blood vessels leading to increased blood
pressure. It also increases the secretion of ADH and aldosterone, and stimulates
the hypothalamus to activate the thirst reflex. Since an overactive renin-angiotensin
system leads to hypertension, renin is an attractive target for the treatment
of this disease. 2-4
Figure 1. Comparison data of 3 renin assay kits. SensoLyte®
520 Human Renin Assay Kit (Cat# 72040), SensoLyte® 390 Renin
Assay Kit (Cat# 72039) and a kit from company B.
Figure 2. A schematic representation of the cleavage of a FRET
peptide substrate by renin is shown on top. The absorption spectrum of QXL™
520 perfectly overlaps with the emission spectrum of 5-FAM (bottom).
SensoLyte® 520 Human Renin Assay Kit
CW. et al. (2012). Bilateral renal ischemia as a model of acute kidney injury
in cats. Res Vet Sc doi.org/10.1016/j.rvsc.2011.12.004.
A. et al. (2011). The (pro)renin receptor is cleaved by ADAM19 in the Golgi
leading to its secretion into extracellular space. Hpertension 34,
May, PC. et al. (2011).
Robust central reduction of amyloid-β in humans with an orally available,
non-peptidic β-secretase inhibitor. J Neurosc 31, 16507.
NP. et al. (2011). High glucose upregulates upstream stimulatory factor 2 in
human renal proximal tubular cells through angiotensin II-dependent activation
of CREB. Exp Nephro doi: 10.1159/000320593.
M. et al. (2011). IQGAP1 regulates ERK1/2 and AKT signaling in the heart and
sustains functional remodeling upon pressure overload. Cardiovasc Res
SensoLyte® 520 Rat Renin Assay Kit
SP. et al. (2012). A novel vascular clip design for the reliable induction of
2-kidney, 1-clip hypertension in the rat. J Appl Physio 112, 362.
W-H. et al. (2012). Renin activates PI3K-Akt-eNOS signaling through the AT1
receptor and Mas receptor to modulate central blood pressure control in the
nucleus tractus solitarii. Brit J Pharma DOI: 10.1111/j.1476-5381.2012.01832.x.
SensoLyte® 520 Mouse Renin Assay Kit
O. et al. (2012). Single atom substitution in mouse protein kinase G eliminates
oxidant sensing to cause hypertension. Nature Med 18, 286.
Renin, Recombinant (5ug)
Prorenin, Recombinant (20 ug)
Renin, Recombinant (20 ug)
390 Renin Assay Kit
390 ACE2 Activity Assay Kit
and Related Peptides
Peptides (ANP, BNP & CNP)
He, FJ. and GA. MacGregor, J. Renin Angiotensin Aldosterone Syst. 4,
2. Wood, JM. et al., Hypertension, 7, 797 (1985).
3. Shibasaki, M. et al., Am. J. Hypertens. 4, 932 (1991).
4. Wood, JM.et al., Biochem. Biophys. Res. Comm. 308, 698 (2003).