Assay Kits

Caspase Activity

Caspases play important roles in apoptosis and cell signaling

Apoptosis is a programmed, cell-autonomous death process. It plays important roles in a variety of physiological and pathological events1 , ranging from normal fetal development to diseases, such as cancer2 , organ failure and neurodegenerative diseases. During apoptosis, caspases execute the disassembly of the cellular components by proteolytic cleavage of a variety of substrates, such as poly-(ADP ribose) polymerase (PARP)3 , DNA-dependent protein kinase (DNA-PK), topoisomerases, and protein kinase C (PKC)δ. 4 At least ten caspases have been discovered. Some of caspases identify and cleave a specific peptide substrate, while others recognize the same peptide substrate4 .



1. Thornberry, N. A. et al. Science 281, 1312-1316 (1998).
2. Reed, J. C. J. Clin. Oncol. 17, 2941-2953 (1999).
3. Lazebnik, Y. A. et al. Nature 371, 346-347 (1994).
4. Villa, P. et al. Trends Biochem. Sci. 22, 388-393 (1997).

Assay Kits for caspase activity monitoring and inhibitors screening

Our Sensolyte® Caspase assay kits use fluorogenic substrates, where the sequence-specific for one or several caspases is conjugated to AFC, AMC or Rh110. Upon protease cleavage, the fluorescence is released proportionally to the caspase activity.

Dye (donor) Donor Ex/Em
SensoLyte® AMC AMC

 351/430 nm

SensoLyte® AFC AFC

 382/480 nm

SensoLyte® Rh110 Rh110

 501/527 nm


The profiling kits contain two 96-well plates (8x12 strips) coated with eight well-known substrates for various caspases, while the Substrate sample kits contain the same peptides, but in bulk.

Two series of 8 peptides are available

8 peptides conjugated with AFC 8 peptides conjugated with AMC

References : AS-71116; AS-71117

SB1 Caspase-1 substrate Ac-YVAD-AFC
SB2 Caspase-1 substrate Ac-WEHD-AFC
SB3 Caspase-2 substrate Ac-VDVAD-AFC
SB4 Caspase-8 substrate Ac-IETD-AFC
SB5 Caspase-3/7 substrate Ac-DEVD-AFC
SB6 Caspase-3/7 substrate Z-DEVD-AFC
SB7 Caspase-9 substrate Ac-LEHD-AFC
SB8 Caspase-6 substrate Ac-VEID-AFC

References : AS-71120; AS-71121

SB1 Caspase-1 substrate Ac-YEVD-AMC
SB2 Caspase-1 substrate Ac-WEHD-AMC
SB3 Caspase-1/4 substrate Ac-YVAD-AMC
SB4 Caspase-3 substrate Ac-DMQD-AMC
SB5 Caspase-3/7 substrate Ac-DEVD-AMC
SB6 Caspase-6 substrate Ac-VEID-AMC
SB7 Caspase-8 substrate Ac-IETD-AMC
SB8 Caspase-9 substrate Ac-LEHD-AMC

  • Caspase Peptides

    Caspase 1, 3, 8 and 9 Substrates and inhibitors

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  • Custom Peptide Synthesis

    We produce standard and complex peptides

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