Peptides

Calpain Substrate, Fluorogenic, (Z-AA)2Rh110 - 5 mg

$124.00
Check your price
  • Cat.Number : AS-60320-5
  • Availability :
    In stock

Select specification

Quantity

(Z-Ala-Ala)2Rh110 is a very sensitive fluorogenic calpain substrate. In general, Rh110-based protease substrates are much more sensitive than the analogous AMC/AFC-based substrate. This enhanced sensitivity might be attributed to the greater fluorescence of the enzymatic product. The colorless and nonfluorescent (Z-Ala-Ala)2Rh110 is hydrolyzed to highly fluorescent rhodamine 110, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments. Alternatively, (Z-Ala-Ala)2Rh110 can also be used to detect calpain in a chromogenic mode since the enzymatic product (rhodamine 110) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). Abs/Em = 497/527 nm

Specifications

Chemistry
Sequence one letter code
  • (Z-AA)2Rh110
Sequence three letter code
  • (Z-Ala-Ala)2Rh110
Molecular Formula
  • C48H47ClN6O11
Molecular Mass/ Weight
  • 885.6
Properties
Absorbance (nm)
  • 497
Emission (nm)
  • 527
Modification
Conjugation type
  • Fluorescent dyes
Modification Name
Conjugation
  • Conjugated
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • Synthetic construct

You may also be interested in the following product(s)

IMG-EN-AS-60844-01.jpg

Calpain Inhibitor Peptide, B27-WT - 0.5 mg

Cat.Number : AS-60844
$124.00 Excl. Tax
IMG-EN-AS-72149-01.jpg

SensoLyte® 520 Calpain Activity Assay Kit Fluorimetric - 1 kit

Cat.Number : AS-72149
$552.00 Excl. Tax
IMG-EN-AS-72150-01.jpg

SensoLyte® AMC Calpain Activity Assay Kit Fluorimetric - 1 kit

Cat.Number : AS-72150
$548.00 Excl. Tax

References

Flow cytometric analysis of protease activities in vital cells.

Biol Chem . 2009 Oct 16 ; 373(2) 547 | DOI : https://doi.org/10.1515/bchm3.1992.373.2.547

  • G. Rothe
  • et al

Real time imaging of calcium-induced localized proteolytic activity after axotomy and its relation to growth cone formation.

Neuron . 1998 Jun 01 ; 20(6) 1123 | DOI : https://doi.org/10.1016/S0896-6273(00)80494-8

  • D. Gitler
  • ME. Spira