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Labeling-detection

CL-APC (Cross Linked-Allophycocyanin) - 1 mg

$81.00
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  • Cat.Number : AS-82002
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    In stock
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Allophycocyanin (APC), a highly fluorescent phycobiliprotein, is made up of alpha and beta subunits and is present as a trimer (αβ)3, which is unstable and susceptible to dissociation at low concentrations. The monomer, αβ, has a lower fluorescence quantum yield compared to the trimer and the maximal absorption is also shifted to 620 nm. The chemically cross-linked APC trimer is much more stable than the native APC trimer, but still retains the same spectroscopic properties as the native APC trimer. APC labeled streptavidin, primary and secondary antibodies have been widely used in applications such as flow cytometry, live cell staining, and immunofluorescent staining.

Specifications

Chemistry
Molecular Mass/ Weight
  • 104000
Properties
Absorbance (nm)
  • 650
Emission (nm)
  • 660
Color
Storage & stability
Form
  • In solution
Resuspension condition
  • Before use, centrifuge the CL-APC suspension at 10,000g for 10 min at 4°C. Discard the supernatant and resuspend the pellet (CL-APC) into the desired buffer. Desalt the sample using either Sephadex G-25 or dialysis.
Storage Buffer
  • Sodium phosphate buffer, pH 7.0 with ammonium sulfate
Storage Conditions
  • Store at 4°C and protect from light. DO NOT FREEZE
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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References

Allophycocyanin 1 as a near-infrared fluorescent tracer: isolation, characterization, chemical modification and use in a homogeneous fluorescence resonance energy transfer system

Anal Biochem . 2001 Sep 15 ; 296(2) 232 | DOI : https://doi.org/10.1006/abio.2001.5298

  • E. Trinquet
  • et al

Time resolved amplification of cryptate emission: a versatile technology to trace biomolecular interactions

J Biotechnol . 2002 Jan 01 ; 82(3) 233 | DOI : https://doi.org/10.1016/S1389-0352(01)00040-X

  • H. Bazin
  • et al