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Protein

Human MMP-8 - 100 µL

$130.00
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  • Cat.Number : AS-72008
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    In stock
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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. MMP-8 (neutrophil collagenase) is synthesized by neutrophils and stored in the specific granules until it is secreted.

Human MMP-8 is a full length pro-enzyme isolated from stimulated human neutrophil granulocytes. Proenzyme can be activated by incubating with 1 mM APMA at 37°C for 1 hr. Its activity can be measured in FRET-based enzymatic assays. 10-20 ng of enzyme is sufficient for FRET-based assay.

Specifications

Chemistry
UniProt number
  • P22894
Molecular Mass/ Weight
  • 85 kDa
Modification
Conjugation
  • Unconjugated
Quantity & Purity
Concentration
  • 10 µg/ml
Purity
  • ≥90% by SDS-PAGE
Storage & stability
Form
  • In solution
Storage Buffer
  • 0.1M Tris, pH 7.5, 0.1M NaCl, 10 mM CaCl2, 0.05% Brij 35, 2mM sodium azide, 1 mg/mL BSA.
Storage Conditions
  • Store at -80°C. Avoid repeated freeze-thaw cycles.
Activity
Application
Biomarker Target
Unit definition
  • One unit of protease hydrolyzes 1 picomole of Mca-Pro-Leu-Gly-LeuDap(Dnp)-Ala-Arg-NH2 per minute at pH 7.5 at 25° C.
Specificity
  • Human MMP-8
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • human

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Citations

Altered expression of matrix metalloproteinases and their tissue inhibitors in matured rat adipocytes in vitro

Biol Res Nurs . 2011 Jun 13 ; 14(3) 2012 | DOI : https://doi.org/10.1177/1099800411410870

  • T. Minematsu