SensoLyte® 520 MMP-8 Assay Kit Fluorimetric - 1 kit
- Cat.Number : AS-71154
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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known. MMP-8 (netrophil collagnease) is proposed as a potential anti-cancer drug target.
SensoLyte® 520 MMP-8 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-8, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
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Citations
Neisseria meningitidis Induces Brain Microvascular Endothelial Cell Detachment from the Matrix and Cleavage of Occludin: A Role for MMP-8
PLoS Pathog . 2010 Apr 29 ; 6(4) e1000874 | DOI : 10.1371/journal.ppat.1000874
- A. Schubert-Unkmeir
Real-time impedance analysis of host cell response to meningococcal infection
J Microbiol Methods. . 2010 Nov 13 ; 84(1) 101 | DOI : 10.1016/j.mimet.2010.11.004
- H. Slanina
Anti-inflammatory and anti-oxidant mechanisms of an MMP-8 inhibitor in lipoteichoic acid-stimulated rat primary astrocytes: involvement of NF-κB, Nrf2, and PPAR-γ signaling pathways.
J Neuroinflam . 2018 Nov 23 ; 15 326 | DOI : https://doi.org/10.1186/s12974-018-1363-6
- E-J. Lee
- et al
Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse.
Mediators Inflamm . 2015 Nov 13 ; 2015 569512 | DOI : https://doi.org/10.1155/2015/569512
- AK. Barton
- et al