SensoLyte® 520 ß-Secretase (BACE1) Activity Assay Kit Fluorimetric - 1 kit
- Cat.Number : AS-71144
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β-Secretase (BACE1) is a membrane-anchored aspartic protease existing in acidic subcellular vesicles. It is a key player in producing β-amyloid peptides through proteolytic cleavage of the β-amyloid precursor protein (APP). Accumulation of neurotoxic β-amyloid peptide is seen in senile plaques in the brains of patients with Alzheimer’s disease (AD), an age-related cognitive disorder. Therefore, targeting β-secretase is considered a potential therapeutic strategy for the prevention and treatment of AD.
The SensoLyte® 520 β-Secretase Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme and for screening of secretase inhibitors. It contains a QXL® 520/ HiLyte™ Fluor 488 FRET substrate. The sequence of this FRET peptide is derived from the β-secretase cleavage site on the Swedish APP mutation. This mutation causes the enhancement of β-secretase to process APP, resulting in an early onset of AD. Active β-secretase cleaves FRET substrate into two separate fragments resulting in the release of HiLyte™ Fluor 488 fluorescence that can be monitored at excitation/emission= 490/520 nm. The long wavelength fluorescence of HiLyte™ Fluor 488 is less interfered by the autofluorescence of components in biological samples and test compounds.
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Citations
Inhibiting β-secretase activity in Alzheimer's disease cell models with single-chain antibodies specifically targeting APP
J Mol Biol . 2011 Jan 14 ; 405 436 | DOI : 10.1016/j.jmb.2010.10.054
- S. Boddapati
Promotion of BACE1 mRNA alternative splicing reduces amyloid β-peptide production
J Biol Chem . 2008 Jul 04 ; 283 18694 | DOI : 10.1074/jbc.M801322200
- K.R. Mowrer
- M.S. Wolfe
Alzheimer's disease protease-containing plasma extracellular vesicles transfer to the hippocampus via the choroid plexus
EBioMedicine . 2022 Mar 01 ; 77 103903 | DOI : https://doi.org/10.1016/j.ebiom.2022.103903
- J-H. Lee
Tongqiaohuoxue Hinders Development and Progression of Atherosclerosis: A Possible Role in Alzheimer’s Disease
Biology . 2020 Oct 27 ; 9(11) 363 | DOI : https://doi.org/10.3390/biology9110363
- E. Ha
- et al
References
Mahanimbine Improved Aging-Related Memory Deficits in Mice through Enhanced Cholinergic Transmission and Suppressed Oxidative Stress, Amyloid Levels, and Neuroinflammation
Brain Sci . 2021 Dec 21 ; 12(1) 12 | DOI : https://doi.org/10.3390/brainsci12010012
- V. Mani
- et al