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Assay-kits

SensoLyte® Anti-a-Synuclein (Human) ELISA Kit - 1 kit

$644.00
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  • Cat.Number : AS-55550-H
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α-Synuclein is a major component of Lewy bodies in the affected neurons in Parkinson’s disease. This protein has a mass of 14.5 kDa (140 amino acids long) and consists of a conserved degenerative amino-terminal domain and an acidic carboxyl-terminal with higher sequence divergence. α-Synuclein is predominantly expressed in brain: specifically in cerebellum, thalamus, neocortex, hippocampus, and striatum regions. Other tissues express α-Synuclein at very low levels. The physiological role of α-Synuclein is not yet well understood. However, the presence of imperfect KTKEGV lipid interacting repeats suggests that it may be involved in synaptic vesicle homeostasis.
This kit is optimized to detect human alpha-Synuclein (α-Synuclein) in cell and tissue lysates, and in human body fluids such as cerebrospinal fluid (CSF) and plasma. Wells are pre-coated with anti-α-Synuclein monoclonal antibodies, blocked, and stabilized for long-term storage. The amount of α-Synuclein is quantified using sandwich ELISA: rabbit polyclonal anti-α-Synuclein specific antibodies directly conjugated to horseradish peroxidase (HRP) are used to detect captured α-Synuclein.

Specifications

Packaging
Kits components
  • Component A: Anti-a-Synuclein: 8-well strip plate (8x12) 12 x 8 well strips Component B: a-Synuclein Standard, human (1 µg/vial): 3 vials Component C: 1X Sample Dilution Buffer: 30 ml Component D: 10X Wash Buffer: 30 ml Component E: TMB Color Substrate Solution: 10 ml Component F: Stop Solution: 10 ml Component G: Detection Antibody Rabbit Polyclonal anti-a-Synuclein IgG-HRP (10 µg/50µl): 50 µl Component H: Adhesive Plate Covers: 2 pieces
Chemistry
UniProt number
  • P37840
Storage & stability
Storage Conditions
  • Store all kit components at 4C for up to 6 months. Protect components E and G from light.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 5 pg/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • human

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Citations

The cleavage effect of mesenchymal stem cell and its derived matrix metalloproteinase-2 on extracellular α-synuclein aggregates in parkinsonian models

Stem cells translational medicine . 2016 Oct 11 ; 6(3) 949-961 | DOI : 10.5966/sctm.2016-0111

  • S.H. Oh

Evidence for α-synuclein prions causing multiple system atrophy in humans with parkinsonism

PNAS . 2015 Sep 22 ; 112(38) E5308-17 | DOI : https://doi.org/10.1073/pnas.1514475112

  • B.S. Prusiner

Propagation of prions causing synucleinopathies in cultured cells

PNAS . 2015 Sep 01 ; 112(35) E4949-58 | DOI : 10.1073/pnas.1513426112

  • A.L. Woerman

Saliva α-Synuclein and A High Extinction Coefficient Protein: A Novel Approach in Assessment Biomarkers of Parkinson's Disease

North Am J Med Sci . 2014 Dec 01 ; 6(12) 633-637 | DOI : 10.4103/1947-2714.147980

  • M.S. Al-Nimer

Changes in amyloid-β and Tau in the cerebrospinal fluid of transgenic mice overexpressing amyloid precursor protein

Sci Transl Med . 2013 Jul 17 ; 5(194) 194re2 | DOI : 10.1126/scitranslmed.3006446

  • L.F. Maia

α-Synuclein and anti-α-synuclein antibodies in Parkinson’s disease, atypical Parkinson syndromes, REM sleep behavior disorder, and healthy controls

PloS One . 2012 Dec 17 ; 7(12) e52285 | DOI : 10.1371/journal.pone.0052285

  • L.M. Smith

Ischemic stroke causes Parkinson’s disease-like pathology and symptoms in transgenic mice overexpressing alpha-synuclein

Acta Neuropatholog Commun . 2022 Feb 24 ; 10 26 | DOI : https://doi.org/10.1186/s40478-022-01327-6

  • S. Lohmann
  • et al

Decreased Exosomal Acetylcholinesterase Activity in the Plasma of Patients With Parkinson's Disease

Front Aging Neurosci . 2021 May 28 ; 13 665400 | DOI : 10.3389/fnagi.2021.665400

  • KH. Shim
  • et al

APOE4 exacerbates α-synuclein pathology and related toxicity independent of amyloid

Sci Transl Med. . 2020 Feb 05 ; 12(529) eaay1809 | DOI : 10.1126/scitranslmed.aay1809

  • N. Zhao
  • et al