Custom Peptide Synthesis
Click Chemistry Peptides
Click chemistry offers one of the fastest and most reliable ways to functionalize peptides, enabling precise conjugation to dyes, PEG, biotin, proteins, or nanoparticles. AnaSpec, manufactures clickable custom peptides from research to GMP grade.
Click Conjugation Advantages
Click Peptide
Manufacturing Capabilities
Flexible Design
N-terminal, C-terminal, or internal side-chain functionalization of your peptide sequence.
Discovery to Clinic
Up to 95% purity for research grade and 98% purity for cleanroom-manufactured GMP grade.
mg to kg
We recognize that flexibility in production scale is important at every stage of your project.
Functional Groups
 |
 |
 |
| Azide | Alkyne | Alkene |
 |
 |
 |
| Aldehyde | Aminooxy | Maleimido |
Click Chemistry Pairs
| Functional Pairs | Reaction Type | Product |
|---|---|---|
| Azide + Alkyne | CuAAC | Triazole |
| Alkene + Thiol | Thiol-Ene | Thioether |
| Aldehyde-Aminooxy | Oxime Ligation | Oxime |
| Aldehyde-Hydrazide | Hydrazone Ligation | Hydrazone |
| Thiol-Maleimide | Maleimide Conjugation | Maleimido |
GMP Grade Click Peptides
ISO 7 classified cleanroom environments Segregation of downstream from upstream processes CMC package for IND support and stability programs
Featured Citations
Development of uMUC-1 Targeted NEMO Particles with pH-Activatable MRI Signals for Enhanced Detection of Malignant Breast Cancer Cells
Panchal, D.M., et al.
ACS Applied Bio Materials 8 Issue 5 (2025) 484–492 DOI 10.1021/acsabm.5c00365
(2-azidoacetic acid-YCAREPPTRTFAYWG) and its scrambled peptide version (2-azidoacetic acid-PRYCGWTEARATPYF) were obtained from AnaSpecFacile Method for the Site-Specific, Covalent Attachment of full-length IgG onto Nanoparticles
Hui, J. Z., et al.
Small 10 Issue 16 (2014) 3354–3363 DOI 10.1002/smll.201303629
DOTA-TOC was produced by AnaspecPost-fabrication Tethering of Molecular Gradients on Aligned Nanofibers of Functional Poly(ε-caprolactone)s
Silantyeva, E.A., et al.
Biomacromolecules 20 Issue 12 (2019) 4494–4501 DOI 10.1021/acs.biomac.9b01264
N3-GRGDS peptide (purity ≥ 95% by HPLC) was purchased from Anaspec
About Click Chemistry
Background
The concept of “click chemistry” was introduced in 2001 by K. Barry Sharpless and colleagues, who coined the term to represent a small set of reliable, high-yielding, modular reactions for linking molecular building blocks.
Copper Catalyzed Peptide Ligation
Among the hallmarks of this approach is the copper(I)-catalyzed azide–alkyne cycloaddition (CuAAC), now a widely adopted prototype click reaction. It enables efficient macrocyclization, bioconjugation and library construction with excellent regio- and chemoselectivity.
Copper-free Click Chemistry Peptides
As residual copper and reducing agents can damage biomolecules, triggering aggregation, degradation, or cytotoxicity, copper-free click approaches were developed. One widely used form is SPAAC, where cyclooctynes (e.g., DIBAC or DIBO) react with azides under ambient, metal-free conditions.
FAQ
Can AnaSpec synthesize my click peptides in either Research or GMP grades?
Yes, AnaSpec can manufacture your click peptide in research grade for discovery use or GMP grade for use in clinical development or as an API starting material.
What click chemistry reactive groups can you add to my peptide?
AnaSpec offers a number of clickable reactive groups to add to your peptide including Azide, Alkyne, Alkene, Aldehyde, Aminooxy, Hydrazide, and Maleimide groups.
Can you further modify my click peptide with unusual building blocks such as D-amino acids or PEG?
Yes, AnaSpec offers an array of unusual non-canonical amino acids and building blocks to further modify your click peptide, including PEG and D-amino acids.
Can I work with clickable peptides in my own lab?
Yes. Click reactions are typically performed under mild, aqueous-compatible conditions that are gentler on biomolecules than traditional organic synthesis reactions. This makes them well-suited for most standard laboratory environments.
