Quenchers
QXL® - Efficient proprietary Quenchers
Quenchers are frequently used in tandem with fluorescent dyes to generate FRET pairs. The most classical FRET pairs are Mca/Dnp and EDANS/DABCYL, both emitting in the blue channel.
Their short absorption wavelengths and low extinction coefficients limit their use as several proteomic samples show background autofluorescence in the blue wavelentgh. QXL® dyes are great choices for you to eliminate these limitations.
Due to the high interest in developing FRET pairs with longer emission wavelengths, AnaSpec has developed the QXL® range of dark quenchers. They are individually optimized to pair with all popular fluorescent dyes such as fluoresceins and rhodamines, as well as our superior HiLyte™ fluor dyes.
Our line of QXL® covers the full visible spectrum with unusually high efficiency.
Our dye/quencher labeling offer
We offer a full-range of dye-quencher FRET pairs and fluorogenic substrates.
The tables below list the dyes and quenchers available to label your custom peptides
| FRET Pairs | ||
|---|---|---|
| Dye (donor) | Quencher (acceptor) | Donor Ex/Em |
| Mca | Dnp |
•
325/393 nm |
| EDANS | DABCYL, DABCYL Plus, QXL®490 |
•
335/493 nm |
| FAM | QXL®520 |
•
492/518 nm |
| FITC | QXL®520 |
•
494/519 nm |
| HiLyte™ Fluor 488 | QXL®520 |
•
502/527 nm |
| CyLyte Fluor 3 | QXL®570 |
•
550/564 nm |
| HiLyte™ Fluor 532 | QXL®570 |
•
545/565 nm |
| HiLyte™ Fluor 555 | QXL®570 |
•
550/566 nm |
| TAMRA | QXL®570 |
•
541/568 nm |
| Rox | QXL®610 |
•
568/591 nm |
| CyLyte Fluor 5 | QXL®670 |
•
648/663 nm |
| HiLyte™ Fluor 647 | QXL®670 |
•
650/675 nm |
| CyLyte Fluor 7 | IR-QXL® |
•
750/773 nm |
QXL®490, 570, 670 are available as stand-alone catalog products, while our most popular QXL®520 and QXL®610 are exclusively available via our custom labeling services.