Custom Peptide Synthesis
Carrier Protein
Peptide Conjugation
AnaSpec offers custom peptide–carrier protein conjugation services for antibody or vaccine development. Common carrier proteins include KLH, BSA, OVA, and CRM197.
Peptides are typically too small for recognition by the immune system to generate a response. Hence they are commonly conjugated to larger carrier protein to stimulate the immune system.
One-Stop Convenience
From synthesis to conjugation, completed in a single order.
Peptide
Expertise
>30 years of expertise in linear, cyclic, and modified peptides.
Carrier Protein Selection
Full panel of carrier proteins including KLH, BSA, cBSA and others.
Our Services
- Peptide synthesis & carrier protein conjugation.
- Pilot optimization study performed to ensure a successful conjugation.
- Additional Options:
Removal of unreacted peptide.
Custom vialing (ie., number of vials, etc).
We are flexible to other custom requests. - Conjugation efficiency on CoA
Featured Citations
Interactions between mouse IgG2 antibodies are common and mediated by plasma C1q
Wood, B.L., Levin G.R.
Cytometry B Clin Cytom 70B (2006) 484–492 DOI 10.1002/cyto.b.20138
"WY-BSA, was synthesized by AnaSpec (San Jose, CA). WY-BSA consists of the dipeptide tryptophan-tyrosine multiply conjugated to bovine serum albumin (BSA)"Panning of a Phage Display Library against a Synthetic Capsule for Peptide Ligands That Bind to the Native Capsule of Bacillus anthracis
Beer, M., Liu, C-Q.
Plos One 7 Issue 9 (2012) DOI: 10.1371/journal.pone.0045472
"synthetic peptides were manufactured and conjugated to BSA by ANASpec"Vaccination against T-cell epitopes of native ApoB100 reduces vascular inflammation and disease in a humanized mouse model of atherosclerosis
Gistera, A. et al.
J. Intern. Med. 281 Issue 4 (2017) 383–397 DOI 10.1111/joim.12589
‘SQVEQFLHRNIQEYLSILTD’ conjugated to keyhole limpet haemocyanin (KLH) protein (Anaspec, San Jose, CA, USA) emulsified in CFA
Carrier Proteins
| Carrier | Example Applications |
|---|---|
| BSA: Bovine Serum Albumin | ELISA, assay controls, hapten presentation, antibody validation |
| cBSA: Cationized Bovine Serum Albumin | Immunization; enhanced peptide loading and uptake; improved immune response vs. native BSA |
| KLH: Keyhole Limpet Hemocyanin | Gold standard for antibody production; strong immune stimulation |
| OVA: Ovalbumin | Standard carrier protein for immunology research; supports reproducible T-cell–dependent immune responses |
| CRM197: Cross-Reacting Material 197 | Vaccine research; controlled, reproducible immune responses |
Conjugation Chemistry
To achieve site-selective and chemoselective conjugation Maleimide–thiol chemistry is our standard method for linking peptides to carrier proteins. The peptide is synthesized to contain a cysteine residue positioned at either the N- or C-terminus of the sequence.
The maleimide-activated carrier protein reacts specifically with the thiol (–SH) group on the cysteine residue to form a stable thioether linkage, ensuring a robust and reliable conjugate.
Alternative conjugation strategies may be available upon consultation.
FAQ
What is conjugation effiency?
It is the percentage of peptide bound to the carrier protein.
What is the difference between BSA and cBSA?
Native BSA has a neutral to slightly negative charge at physiological pH. cBSA is chemically modified with primary amines, resulting in a strong net positive charge.
What carrier proteins can you conjugate to peptides?
Yes, we can individually conjugate your peptide to several carrier proteins. For example, we can conjugate 1mg peptide to BSA and another 1mg peptide to KLH, and deliver each in a separate vial.
How is my peptide-carrier protein conjugate shipped?
The conjugated peptide is shipped on blue ice.
How should I store my peptide-carrier protein conjugate?
Store at 2-8°C for a few days or -20°C for longer term, avoiding repeat freeze-thaw cycles.
