IMG-EN-AS-22991-01.jpg
Peptides

HCV Protease FRET Substrate (RET S1)

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  • Cat.Number : AS-22991
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This peptide is a HCV protease substrate incorporating an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm.

Specifications

Chemistry
Sequence one letter code
  • Ac-DE-D(Edans)-EE-Abu-ψ-[COO]-AS-K(Dabcyl)-NH2
Sequence three letter code
  • Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-ψ-[COO]-Ala-Ser-Lys(DABCYL)-NH2
Molecular Mass/ Weight
  • 1548.6
Properties
Absorbance (nm)
  • 355
Emission (nm)
  • 500
Modification
Conjugation type
  • Double dyes
Modification Name
Conjugation
  • Conjugated
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Storage Conditions
  • - 20 °C Protected from light
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • HCV

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Citations

Hepatitis C Virus NS2 Protein Contributes to Virus Particle Assembly via Opposing Epistatic Interactions with the E1-E2 Glycoprotein and NS3-NS4A Enzyme Complexes

J Virol. . 2009 Jun 10 ; 83(17) 8379 | DOI : 10.1128/JVI.00891-09

  • T. Phan

References

A Continuous Assay of Hepatitis C Virus Protease Based on Resonance Energy Transfer Depsipeptide Substrates

Anal Biochem . 1996 Aug 15 ; 240(1) 60 | DOI : https://doi.org/10.1006/abio.1996.0331

  • M. Taliani

A high throughput assay of the hepatitis C virus nonstructural protein 3 serine proteinase

J. Virol. Methods . 1999 Jun 01 ; 80(1) 77 | DOI : https://doi.org/10.1016/S0166-0934(99)00034-8

  • N. Kakiuchi
  • et al

Use of a Fluorescence Plate Reader for Measuring Kinetic Parameters with Inner Filter Effect Correction

Anal. Biochem. . 1999 Feb 15 ; 267(2) 331 | DOI : https://doi.org/10.1006/abio.1998.3014

  • Y. Liu
  • et al