Assay-kits

SensoLyte® Anti-Human β-Amyloid (1-42) Quantitative ELISA Kit *Colorimetric*

$566.00
Check your price
  • Cat.Number : AS-55552
  • Availability :
    In stock
  • Shipping conditions : Ice fees will apply

Select specification

Quantity

This SensoLyte® high-sensitivity (2 pg/ml) beta-Amyloid (1-42) Quantitative ELISA Kit (Human) is optimized to detect human beta-Amyloid (1-42) peptide in human brain lysate, transgenic mouse brain lysate, human cerebrospinal fluid, plasma, or saliva. Wells are pre-coated with monoclonal anti-beta-Amyloid (1-42) specific capture antibodies and blocked with a proprietary blocking solution. The amount of human beta-Amyloid (1-42) is quantified using ELISA. Ample materials and reagents are provided to perform 96 assays.

Alzheimer's Disease (AD) is the most common neurodegenerative disorder in elderly people. It has been demonstrated that AD has biological causes and is characterized by the presence of senile plaques and neurofibrillary tangles mainly in cerebral cortex and hippocampus brain regions. Beta-Amyloid (1-40) (Aα40) and beta-Amyloid (1-42) (Aα42) are the main components of the above plaques; however, other forms of beta-Amyloid peptides are also present. Both peptides are cleaved from the Amyloid Precursor Protein (APP) by α-secretase and γ-secretase enzymes. Many studies suggest that Aα42 or/and Aα43 are required to initiate formation of amyloid plaques and neurofibrills that leads to the neurodegeneration, while Aα40 is less neurotoxic.

Specifications

Packaging
Kits components
  • Component A: Mouse monoclonal anti-beta-Amyloid (1-42) coated plate: 12 x 8-well strips. Component B: Human beta-Amyloid (1-42) Peptide Standard (lyophilized): 1 µg. Component C: 1 X Sample Dilution Buffer: 60 ml. Component D: 10 X Wash Buffer: 30 ml. Component E: TMB Color Substrate Solution: 10 ml. Component F: Stop Solution: 10 ml. Component G: Peptide Standard Reconstitution Buffer: 1 ml. Component H: Detection Antibody (Rabbit anti-Human beta-Amyloid-HRP): 50 µl. Component I: 1 X Detection Antibody Dilution Buffer: 10 ml. Component J: Adhesive Plate Covers: 2 pieces.
Chemistry
UniProt number
  • P05067
Storage & stability
Storage Conditions
  • Store all components at 4°C for up to 6 months. Component B Reconstituted Peptide Standard must be stored at -80 °C in small aliquots. Protect components E and G from light.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 2 pg/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • human

You may also be interested in the following product(s)

IMG-EN-AS-20276-01.jpg

Beta-Amyloid (1-42)

Cat.Number : AS-20276
$341.00 Excl. Tax

Citations

Amyloid precursor protein products concentrate in a subset of exosomes specifically endocytosed by neuron

Cell. Mol. Life Sci. . 2018 Feb 01 ; 75(4) 757 | DOI : 10.1007/s00018-017-2664-0

  • K. Laulagnier
  • et al

Alteration of High-Density Lipoproteins functionality in Alzheimer’s disease patients

Canadian J Physiol Pharmacol . 2017 Aug 01 ; 95(8) 894 | DOI : 10.1139/cjpp-2016-0710

  • P. Camponova
  • et al