The recombinant human a-synuclein (1-140) (GenBank Accession # NP_000336) was expressed and purified from E. coli and conjugated with the fluorescence dye HiLyte FluorTM 488. This protein is produced without affinity tag.
Parkinson's disease is predominantly a movement disorder resulting from degeneration of dopaminergic neurons in the substantia nigra. The cause of the disease is unknown, but substantial evidence suggests that the aggregation of a-synuclein is a critical step in the etiology of Parkinson's disease (PD). a-Synuclein is an abundant brain protein of 140 residues that is present in high concentrations at presynaptic terminals and is found in both soluble and membrane-associated fractions of the brain. Several possible functions have been suggested, and it appears to be involved in vesicle release and trafficking. In vitro incubation of a-synuclein in the presence of certain amounts of salt (i.e. 0.1M NaCl) with agitation can form fibril structure.
HiLyte FluorTM 488 labeled human a-synuclein is supplied frozen at 1 mg/ml in 10 mM sodium phosphate buffer (pH=7.0). Store at 2-4 ºC for immediate use within 1 week or at –80 ºC for up to 12 months. Keep in dark and avoid repeated freeze-thaw cycles.
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Green fluorescence. Excitation/Emission wavelengths= 490nm/525nm