Existing Account

Please login first to complete purchase/ quotation request, view custom order reports, or create favorites list.

Customer ID:
Stay Logged In

Forgot your Customer ID or Password?
New Account

Don't have an account with us yet? Please set up an account to place order or obtain customer services.

Assay Kits  >  Phosphatase Assay Kits  >>  SensoLyte® pNPP Alkaline Phosphatase Assay Kit *Colorimetric*

Product Name SensoLyte® pNPP Alkaline Phosphatase Assay Kit *Colorimetric*
Size 1 kit
Catalog # AS-72146
US$ $173

The change of alkaline phosphatase activity is involved in a variety of physiological and pathological events, such as bone development, bone-related diseases, gestation-related diseases and inflammatory bowel diseases. Alkaline phosphatase is also a popular enzyme conjugated with secondary antibody for ELISA. p-Nitrophenyl phosphate (pNPP) is proven to be an effective chromogenic substrate for alkaline phosphate. This SensoLyte® kit can be used to detect alkaline phosphatase activity in biological samples and alkaline phosphatase-based ELISA. The kit contains: pNPP chromogenic liquid substrate (absorption maximum = 405 nm upon dephosphorylation), Assay buffer, Stop solution, Alkaline phosphatase standard, “mix and read” assay protocol that is compatible with high throughput screening.

Kit size: 500 assays

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Product Citations Knopp, M. et al. (2015). Amelioration of the fitness costs of antibiotic resistance due to reduced outer membrane permeability by upregulation of alternative porins MolBioEvol 32 doi:10.1093/molbev/msv195

Dai, J. et al. (2014). Cabozantinib Inhibits Prostate Cancer Growth and Prevents Tumor-Induced Bone Lesions. Clin Cancer Res 20, 617. doi: 10.1158/1078-0432.CCR-13-0839.

Okon, IS. et al. (2014). Liver kinase B1 expression promotes phosphatase activity and abrogation of receptor tyrosine kinase phosphorylation in human cancer cells. J Biol Chem 289, 1639.

Mah, YJ. et al. (2014). The effect of epigallocatechin-3-gallate (EGCG) on human alveolar bone cells both in vitro and in vivo. Archives Oral Biol 59, 539.

Pauksch, L. et al. (2014). Biocompatibility of silver nanoparticles and silver ions in primary human mesenchymal stem cells and osteoblasts. Acta Biomat 10, 439.

Rebollo, A. et al. (2014). Liquid fructose downregulates Sirt1 expression and activity and impairs the oxidation of fatty acids in rat and human liver cells. Biochim Biophys Acta -Mol Cell Biol Lipids 1841, 514.

Yamano, S. et al. (2014). The effect of a bioactive collagen membrane releasing PDGF or GDF-5 on bone regeneration. Biomat 35, 2446.

Choi, B. et al. (2012). Facile surface functionalization with glycosaminoglycans by direct coating with mussel adhesive protein. Tissue Eng Part C Meth 18, 71.

Kamiyama, M. et al. (2012). The establishment of a primary culture system of proximal tubule segments using specific markers from normal mouse kidneys. Intl J Mol Sci 13, 5098. doi:10.3390/ijms13045098

Kim, S-M. et al. (2012).Gelatin-layered and multi-sized porous β-tricalcium phosphate for tissue engineering scaffold. Nanoscale Res Lett 7, 78. doi:10.1186/1556-276X-7-78Open Access

Martinez-Conesa, E. et al. (2012). Characterization of ocular surface epithelial and progenitor cell markers in human adipose stromal cells derived from lipoaspirates. Invest. Ophthalmol. Vis. Sci 53, 513-520.

Sola, A. et al. (2012). Heat treatment of Na2O-CaO-P2O5-SiO2 bioactive glasses: Densification processes and postsintering bioactivity. J Biomed Mat Res 100A, 305. doi:10.1002/jbm.a.33276

Sun, J. et al. (2012). Biomimetic interpenetrating polymer network hydrogels based on methacrylated alginate and collagen for 3D pre-osteoblast spreading and osteogenic differentiation. Soft Matter 8, 2398.

Sunada, K. et al. (2012). Highly efficient antiviral and antibacterial activities of solid-state cuprous compounds. J Haz Mat 235-236, 265. doi:10.1002/jbm.a.33276

Thorpe, M. et al. (2012). The external mechanical environment can override the influence of local substrate in determining stem cell fate. J Biomechanics 45, 2483.

Vinardell, T. et al. (2012). Hydrostatic pressure acts to stabilize a chondrogenic phenotype in porcine joint tissue derived stem cells. Euro Cells and Mat 23, 121.

Williams, C. et al. (2012). A comparison of human smooth muscle and mesenchymal stem cells as potential cell sources for tissue-engineered vascular patches. Tissue Eng Part A.

Abedini, S. et al. (2011). Effects of cryopreservation with a newly-developed magnetic field programmed freezer on periodontal ligament cells and pulp tissues. Cryobiol 10.1016/j.cryobiol.2011.03.001.

Brammer, K. et al. (2011). Highly bioactive 8 nm hydrothermal TiO2 nanotubes elicit enhanced bone cell response. Adv Eng Mat 13, B88.

Brammer, K. et al. (2011). Comparative cell behavior on carbon-coated TiO2 nanotube surfaces for osteoblasts vs. osteo-progenitor cells. Acta Biomaterialia 7, 2697.

Frandsen, C. et al. (2011). Zirconium oxide nanotube surface prompts increased osteoblast functionality and mineralization. Mat Sci and Eng 31, 1716.

Guller, S. et al. (2011). Protein composition of microparticles shed from human placenta during placental perfusion: Potential role in angiogenesis and fibrinolysis in preeclampsia. Placenta 32, 63.

Hong, D. et al. (2011). Quantitative proteomic analysis of dexamethasone-induced effects on osteoblast differentiation, proliferation, and apoptosis in MC3T3-E1 cells using SILAC. Osteo Inter 22, 2175.

Jeon, O. et al. (2011). Affinity-based growth factor delivery using biodegradable, photocrosslinked heparin-alginate hydrogels. J of Cont Rel 154, 258.

Kemoun, P. et al. (2011). The role of cell surface markers and enamel matrix derivatives on human periodontal ligament mesenchymal progenitor responses in vitro. Biomat 32, 7375.

Kulp, A. et al. (2011). Recognition of β-Strand motifs by RseB is required for E activity in escherichia coli. J. Bacteriol 193, 6179-6186.

Lau, E. et al. (2011). Effect of low-magnitude, high-frequency vibration on osteogenic differentiation of rat mesenchymal stromal cells. J of Ortho Res 29, 1075.

Lin, F. et al. (2011). Role of mitogen-activated protein kinase in osteoblast differentiation. J Ortho Res 29, 204.

Manning, A. et al. (2011). Contribution of bacterial outer membrane vesicles to innate bacterial defense Biomedcentral 11, 258.

McNeil, S. et al. (2011). Polycaprolactone fibres as a potential delivery system for collagen to support bone regeneration. Curr Drug Deliv 8, 448.

Nazari-Shafti, T. et al. (2011). Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection. Retrovirol 8, 3.

Oh, P-S. et al. (2011). Schlafen-3 decreases cancer stem cell marker expression and autocrine/juxtacrine signaling in FOLFOX-resistant colon cancer cells. AJP-GI 10.1152/ajpgi.00403.

Rathbone, C. et al. (2011). Effect of various concentrations of antibiotics on osteogenic cell viability and activity. J Orthopaedic Res 10.1002/jor.21343.

Salerno, A. et al. (2011). Processing/structure/property relationship of multi-scaled PCL and PCL–HA composite scaffolds prepared via gas foaming and NaCl reverse templating. Biotech and Bioengin 108, 963.

Shah, D. et al. (2011). Cell invasion of poultry-associated Salmonella Enteritidis isolates is associated with pathogenicity, motility and secretion of type-three secretion system secreted proteins. Microbiol 10.1099/mic.0.044461-0.

Tan, F. et al. (2011). Osteoconductivity and growth factor production by MG63 osteoblastic cells on bioglass-coated orthopedic implants. Biotech and Bioengin 108, 454.

Uhrig, R. et al. (2011). Two ancient bacterial-like PPP family phosphatases from arabidopsis are highly conserved plant proteins that possess unique properties. Plant Physiology 157, 1778-1792.

Wang, J. et al. (2011). The effect of scaffold architecture on odontogenic differentiation of human dental pulp stem cells. Biomaterials 32, 7822.

Yamano, S. et al. (2011). Bioactive collagen membrane as a carrier for sustained release of PDGF. J Tis Sci Eng 2, 4.

Zhou, Q. et al. (2011). Localized colonic stem cell transplantation enhances tissue regeneration in murine colitis. J Cell Mol Med

Barbier de La Serre, C. et al. (2010). Propensity to high-fat diet-induced obesity in rats is associated with changes in the gut microbiota and gut inflammation. Am J Physiol Gastrointest Liver Physiol 299, G440.

Choi, B. et al. (2010). Cell behavior on extracellular matrix mimic materials based on mussel adhesive protein fused with functional peptides. Biomaterials 31, 8980.

Cramer, C. et al. (2010). Persistent high glucose concentrations alter the regenerative potential of mesenchymal stem cells. Stem Cells Dev 19, 1875.

Fan, Q. et al. (2010). Requirement of TGFβ signaling for SMO-mediated carcinogenesis. J Biol Chem 285, 36570.

Hildreth, B. et al. (2010). PTHrP 1-141 and 1-86 increase in vitro bone formation. J Surg Res 162, e9.

Hong, D. et al. (2010). Morphological and proteomic analysis of early stage of osteoblast differentiation in osteoblastic progenitor cells. Experimental Res 316, 2291.

Hu, J. et al. (2010). Response of human embryonic stem cells derived mesenchymal stem cells to osteogenic factors and architectures of materials during in vitro osteogenesis. Tissue Engineer Part A 10.1089/ten.TEA.2010.0097.

Kanwar, S. et al. (2010). The Wnt/beta-catenin pathway regulates growth and maintenance of colonospheres. Mol Cancer 6, 212.

Kemmis, C. et al. (2010). Bone morphogenetic protein 6 drives both osteogenesis and chondrogenesis in murine adipose-derived mesenchymal cells depending on culture conditions. Biochem Biophys Res Commun. 401, 20.

Lu, C. et al. (2010). MT1-MMP controls human mesenchymal stem cell trafficking and differentiation. Blood 115, 221.

Misra, S. et al. (2010). Effect of nanoparticulate bioactive glass particles on bioactivity and cytocompatibility of poly(3-hydroxybutyrate) composites. J R Social Interface 7, 453.

Oku, S. et al. (2010). JAK2 V617F uses distinct signalling pathways to induce cell proliferation and neutrophil activation. British journal of Haematology 150, 334.

Raucci, MG. et al. (2010). Biomineralized porous composite scaffolds prepared by chemical synthesis for bone tissue regeneration. Acta Biomater 10.1016/j.actbio.2010.04.018.

Thi, MM. et al. (2010). Characterization of hTERT-immortalized osteoblast cell lines generated from wild-type and connexin43-null mouse calvaria. Am J Physiol Cell Physiol 299, C994.

Wang, J. et al. (2010). The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(l-lactic acid) scaffolds in vitro and in vivo. Acta Biomaterialia 6, 3856.

Zhang, Z. et al. (2010). A comparison of bioreactors for culture of fetal mesenchymal stem cells for bone tissue engineering. Biomaterials 31, 8684.

Hemauer, S. et al. (2009). Opiates inhibit paclitaxel uptake by P-glycoprotein in preparations of human placental inside-out vesicles. Biochem Pharm 78, 1272.

Kurimoto-Nakano, R. et al. (2009). Replicative senescence of vascular smooth muscle cells enhances the calcification through initiating the osteoblastic transition. Am J Physiol Heart Circ Physiol 297, H1673-H1684

Lee, J. et al. (2009). Modular peptides promote human mesenchymal stem cell differentiation on biomaterial surfaces. Acta Biomater 6, 21.

Pennisi, A. et al. (2009). Inhibitor of DASH proteases affects expression of adhesion molecules in osteoclasts and reduces myeloma growth and bone disease. British J of Hematology 145, 775.

Stanisic, V. et al. (2009). OTU domain-containing ubiquitin aldehyde-binding protein 1 (OTUB1) deubiquitinates estrogen receptor (ER) α and affects ERα transcriptional activity. J Biol Chem 284, 16135.

Zhang, Z. et al. (2009). Superior osteogenic capacity for bone tissue engineering of fetal compared with perinatal and adult mesenchymal stem cells. Stem Cells 27, 126.

Haraguchi, N. et al. (2008). CD133+CD44+ population efficiently enriches colon cancer initiating cells. Ann Surg Oncol 15, 2927.

Meng, Q. et al. (2008). Regulating the age-related oxidative damage, mitochondrial integrity, and antioxidative enzyme activity in fischer 344 rats by supplementation of the antioxidant epigallocatechin-3-gallate. Rejuv Res 11, 649.

Scaffidi, P. et al. (2008). Lamin A-dependent misregulation of adult stem cells associated with accelerated ageing. Nature Cell Biol 10, 452.

Bae, J. et al. (2007). Engineering a disulfide bond to stabilize the calcium binding loop of activated protein C eliminates its anticoagulant but not protective signaling properties. J Biol Chem 10.1074/jbc.M610547200.

Yang, L. et al. (2007). Identification of a specific exosite on activated protein C for interaction with protease-activated receptor 1. J Biol Chem 282, 25493.
  < Back