Phophatases hydrolyze a variety of organic phosphate monoesters to alcohols and inorganic phosphate, and have quite a few subfamilies. They are an important class of enzymes that are involved in cellular signal transduction, cell metabolism and other biological activities. Some phosphatases serve as enzyme markers. For example, PALP-1
, the gene for human placental alkaline phosphate, has been used as a eukaryotic receptor gene that is superior to IacZ
for lineage studies in murine retina. This gene has also been engineered to produce a secreted alkaline phosphatase (SEAP), allowing quantitation of gene expression without disrupting the cells. Some phosphatases (such as protein phosphatase) are identified as drug screening targets.
AnaSpec offers a variety of highly purified chromogenic and fluorogenic substrates for detecting alkaline phosphatase activities in solutions, in cell extracts, and in live cells; as well as, detecting alkaline phosphatase activity either in a fluorogenic or colorimetric mode. FDP is widely used to detect other phosphatase activity such as protein phosphatase PTB1. Our SensoLyte®
ELISA alkaline phosphatase assay kits provide both sensitivity and convenience. It has been used to detect as low as atto mole (10-18
mole) of primary antibodies in an ELISA format. The kit has also been used to detect alkaline phosphatase in other media.Click here
to view and download our Phosphatase Assay flyer in PDF format.