AggreSureÖ β-Amyloid 40 & 42 Peptides
AnaSpec beta-Amyloid Aggresure Peptides and Assay Kits

AggreSure TM Beta-Amyloid 1-42 & 1-40 Peptides, tested and guaranteed for aggregation are intended for the study of Alzheimer's disease pathology Read more...

Applications

  • Monitoring amyloid aggregation
  • Amyloid aggregation kinetics
  • Fibril formation
  • Amyloid inhibitor screening

Features & Benefits

  • Guaranteed for aggregation
  • Ready to use for direct fibrillation studies
  • Net Peptide quantities for better accuracy
  • In-house proprietary pre-treatment to ensure high % of monomeric state
  • Save time with our improved formulation
  • Cited in high-impact journals

Fig 1. TEM of Aβ42 fibrils (AS-72216)

Product Name Catalog # Size á
AggreSureᵀᴹ β-Amyloid (1-42), Human
AS-72216
0.25 mg Net Peptide
AggreSureᵀᴹ β-Amyloid (1-40), human
AS-72215
0.25 mg Net Peptide

Why study Amyloid Aggregation?

Hallmarks of Alzheimer's neurodegenerative pathology include the production, oligomerization/fibrillation, and deposition of beta-amyloid peptide. Drug discovery for compounds that affect amyloid aggregation are being explored as leads for AD treatment, making it important to have reliable tools such as Aggresure to study amyloidoses.

How is aggregation guaranteed with our AggreSureTM Peptides?

AggreSureTM beta-amyloid (1-42, 1-40) peptides are pre-treated and tested for aggregation using SensoLyte« ThT Aβ42 or Aβ40 Aggregation kits respectively.

Fibrillation measurement of Aβ42 over time using
Thioflavin T Aggregation kit. Cat# AS-72214

Fig 2. Fluorescence signal is correlated with an increase of A▀42 fibril formation. Phenol Red, Morin, Tannic Acid, Dopamine, and o-Vanillin were added at 100ÁM final concentration to inhibit A▀42 aggregation. Fluorescence was monitored at Ex/Em= 440/484 nm every 5 minutes at 37║C with 15 seconds shaking between reads (Flexstation II-384 fluorimeter, Molecular Devices).

Fibrillation measurement of Aβ40 over time using
Thioflavin T Aggregation kit. Cat# AS-72213

Fig 3. Fluorescence signal is correlated with an increase of A▀40 fibril formation. Phenol Red, Morin, Rhodamine B, and Tannic Acid were added at 50 ÁM final concentration to inhibit A▀40 aggregation. Fluorescence was monitored at
Ex/Em= 440/484 nm every 5 minutes at 37║C with 15 seconds shaking between reads (Flexstation II-384 fluorimeter, Molecular Devices).


Trending Citations

Cheng, Q. et al (2018). TREM2-activating antibodies abrogate the negative pleiotropic effects of the Alzheimer's disease variant TREM2(R47H) on murine myeloid cell function. J Biol Chrem 293(32):12620-12633. doi: 10.1074/jbc.RA118.001848.

Reinhardt, S. et al. (2018). Identification of disulfiram as a secretase-modulating compound with beneficial effects on Alzheimer's disease hallmarks. Sci Rep 8(1):1329. doi: 10.1038/s41598-018-19577-7.

Maneshi, MM. et al. (2018). Enantiomeric Aβ peptides inhibit the fluid shear stress response of PIEZO1. Sci Rep 8(1):14267. doi: 10.1038/s41598-018-32572-2.

Livadiotis, G. et al (2017). Experimental Analysis of Interacting HT22 Plasma Mrembrane Cholesterol and β-Amyloid. Advances in Alzheimer's Disease 6(04):75.

An, S. et al (2017). Dynamic changes of oligomeric amyloid β levels in plasma induced by spiked synthetic Aβ42. Alzheimers Res Ther 9:86. doi:10.1186/s13195-017-0310-6

Wang, M. et al (2017). Oligomeric forms of amyloid β protein in plasma as a potential blood-based biomarker for Alzheimer's disease. Alzheimers Res Ther 9:98. doi:10.1186/s13195-017-0324-0

Hartz. AM, et al. (2016). Aβ40 Reduces P-Glycoprotein at the Blood-Brain Barrier through the Ubiquitin-Proteasome Pathway. J Neurosci. 36(6):1930-41. doi:10.1523/JNEUROSCI.0350-15.2016.