Assay Kits
Protease & Enzyme Activity
AnaSpec has extensive experience developing Protease and Enzyme Activity
Assay Kits - since 2004. By leveraging our proprietary FRET long-wavelength quencher and dye technologies, we develop and select only the most efficient One-Step assays to place on the market. This portfolio of kits detects a variety of disease targets in different types of
biological samples.
Convenient
Our Assay kits are convenient for general research or high throughput screening for the discovery of enzyme inhibitors or enzyme activators.
Sensitive
Our FRET assay kits and Fluorogenic assay kits are highly sensitive with high signal-to-background ratios and are tested for quality.
Adaptable
Our homogenous mix-and-read enzyme activity kits are available as off-the-shelf catalog products but can also be optimized for your specific application. Bulk quantities available.
Our collection of Activity Assay Kits
Category | Products | |
Acetylcholinesterase | SensoLyte® 520 Acetylcholinesterase Assay Kit Fluorimetric | Order Now |
ADAM | SensoLyte® 520 ADAM10 Activity Assay Kit Fluorimetric | Order Now |
SensoLyte® 520 TACE (a-Secretase, ADAM17) Activity Assay Kit Fluorimetric | Order Now | |
ADAMTS | SensoLyte® 520 ADAMTS13 Activity Assay Kit Fluorimetric | Order Now |
SensoLyte® 520 Aggrecanase-1 Assay Kit Fluorimetric | Order Now | |
ACE 2 (Angiotensin-Converting Enzyme 2) | SensoLyte® 390 ACE2 Activity Assay Kit Fluorimetric | Order Now |
Calpain | SensoLyte® 520 Calpain Activity Assay Kit Fluorimetric | Order Now |
SensoLyte® AMC Calpain Activity Assay Kit Fluorimetric | Order Now | |
Caspases | SensoLyte® Caspase Activity Assay Kits | Order Now |
Cathepsins | SensoLyte® Cathepsin Activity Assay Kits | Order Now |
Deubiquitination | SensoLyte® 520 Deubiquitination Assay Kit Fluorimetric | Order Now |
SensoLyte® 440 Deubiquitination Assay Kit Fluorimetric | Order Now | |
Dipeptidyl Prolyl Isomerase 4 (DPPIV) | SensoLyte® Rh110 DPPIV Assay Kit Fluorimetric | Order Now |
SensoLyte® AMC DPPIV Assay Kit Fluorimetric | Order Now |
Category | Products | |
Elastase | SensoLyte® Green Elastase Assay Kit Fluorimetric | Order Now |
SensoLyte® Rh110 Elastase Assay Kit Fluorimetric | Order Now | |
ECE (Endothelin Converting Enzyme) | SensoLyte® 520 ECEs Activity Assay Kit Fluorimetric | Order Now |
Enterokinase | SensoLyte® 520 Enterokinase Assay Kit Fluorimetric | Order Now |
SensoLyte® Rh110 Enterokinase Assay Kit Fluorimetric | Order Now | |
Factor Xa | SensoLyte® 520 Factor Xa Assay Kit Fluorimetric | Order Now |
SensoLyte® Rh110 Factor Xa Assay Kit Fluorimetric | Order Now | |
Furin | SensoLyte® Rh110 Furin Activity Assay Kit Fluorimetric | Order Now |
GBA (Glucocerebrosidase) | SensoLyte® Blue Glucocerebrosidase (GBA) Activity Assay Kit Fluorimetric | Order Now |
SensoLyte® Red Glucocerebrosidase (GBA) Activity Assay Kit Fluorimetric | Order Now | |
Glutaminyl cyclase | SensoLyte® Green Glutaminyl Cyclase Activity Assay Kit Fluorimetric | Order Now |
Granzymes A & B | SensoLyte® 520 Granzyme A Activity Assay Kit, Fluorimetric | Order Now |
SensoLyte® 520 Granzyme B Activity Assay Kit, Fluorimetric | Order Now | |
Glutathione-S-Transferase (GST) | SensoLyte® GST Activity Assay Kit Fluorimetric | Order Now |
Category | Products | |
HDAC (Histone deacetylase) | SensoLyte® 520 HDAC Activity Assay Kit Fluorimetric | Order Now |
HAT (Histone acetyltransferase) | SensoLyte® HAT (p300) Assay Kit Fluorimetric | Order Now |
HCV Protease | SensoLyte® 490 HCV Protease Assay Kit Fluorimetric 500 assays | Order Now |
SensoLyte® 490 HCV Protease Assay Kit Fluorimetric 200 assays | Order Now | |
SensoLyte® 520 HCV Protease Assay Kit Fluorimetric | Order Now | |
HIV Protease | SensoLyte® 490 HIV Protease Assay Kit Fluorimetric | Order Now |
SensoLyte® 520 HIV Protease Assay Kit Fluorimetric | Order Now | |
Insulin Degrading Enzyme (IDE) | SensoLyte® 520 IDE Activity Assay Kit Fluorimetric | Order Now |
Kallikrein | SensoLyte® Rh110 Plasma Kallikrein Activity Assay Kit Fluorimetric | Order Now |
Category | Products | |
Matriptase | SensoLyte® Rh110 Matriptase Activity Assay Kit Fluorimetric | Order Now |
Matrix Metalloproteinases (MMPs) | SensoLyte® MMP Activity Assay Kits | Order Now |
Meprins | SensoLyte® 520 Meprin β Activity Assay Kit Fluorimetric | Order Now |
SensoLyte® 520 Meprin α Activity Assay Kit Fluorimetric | Order Now | |
Neprilysin | SensoLyte® 520 Neprilysin Activity Assay Kit Fluorimetric | Order Now |
Peptidyl-Prolyl Isomerase (Pin1) | SensoLyte® Green PIN1 Activity Assay Kit Fluorimetric | Order Now |
Peroxidase | SensoLyte® Peroxidase Assay Kits | Order Now |
Phosphatase | SensoLyte® Phosphatase Assay Kits | Order Now |
Plasmin | SensoLyte® AFC Plasmin Activity Assay Kit Fluorimetric | Order Now |
SensoLyte® Rh110 Plasmin Activity Assay Kit Fluorimetric | Order Now | |
Plasminogen Activator | SensoLyte® AMC Tissue-type (tPA) Activity Assay Kit Fluorimetric | Order Now |
SensoLyte® AFC Urokinase (uPA) Activity Assay Kit Fluorimetric | Order Now | |
Protease-Generic | SensoLyte® Green Protease Assay Kit Fluorimetric | Order Now |
SensoLyte® Red Protease Assay Kit *Fluorimetric | Order Now |
Category | Products | |
Renin | SensoLyte® Renin activity assay kits | Order Now |
SARS-CoV-2 Proteases | SensoLyte® SARS-CoV-2 3CL Protease Activity Assay Kit Fluorimetric | Order Now |
SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit Fluorimetric | Order Now | |
Secretases | SensoLyte® 520 β-Secretase (BACE1) Activity Assay Kit Fluorimetric | Order Now |
SensoLyte® 520 BACE2 Activity Assay Kit Fluorimetric | Order Now | |
SensoLyte® 520 TACE (a-Secretase) Activity Assay Kit Fluorimetric | Order Now | |
Sirtuin (SIRT) | SensoLyte® 520 FRET SIRT1 Assay Kit Fluorimetric | Order Now |
SensoLyte® Green SIRT1 Assay Kit Fluorimetric | Order Now | |
SensoLyte® 520 FRET SIRT2 Assay Kit Fluorimetric | Order Now | |
SensoLyte® Green SIRT2 Assay Kit Fluorimetric | Order Now | |
Sortase A | Sortase A | Order Now |
Thrombin | SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric | Order Now |
SensoLyte® AFC Thrombin Activity Assay Kit Fluorimetric | Order Now | |
Tobacco Etch Virus (TEV) Protease | SensoLyte® 520 TEV Protease Assay Kit Fluorimetric | Order Now |
Transglutaminase | SensoLyte® Red Transglutaminase Assay Kit Fluorimetric | Order Now |
SensoLyte® Transglutaminase Activity Assay Kit Colorimetric | Order Now | |
West Nile Virus (WNV) Protease | SensoLyte® 440 West Nile Virus Protease Assay Kit Fluorimetric | Order Now |
SensoLyte® 570 West Nile Virus Protease Assay Kit Fluorimetric | Order Now |
FRET substrates
FRET occurs between a peptide tagged to a donor and an acceptor when placed within 10-100Å of each other
resulting in the donor’s excitation fluorescence to be quenched by the acceptor.
Enzymatic hydrolysis of the peptide results in recovery of the donor fluorescence following spatial separation of the donor and acceptor upon energy transfer.
Dye (donor) | Quencher (acceptor) | Donor Ex/Em | |
SensoLyte® 390 | Mca | Dnp |
•
325/393 nm |
---|---|---|---|
SensoLyte® 490 | EDANS | DABCYL |
•
340/490 nm |
SensoLyte® 520 | 5-FAM or HiLyte™ Fluor 488 | QXL®520 |
•
494/521 nm |
SensoLyte® 570 | 5-TAMRA | QXL®570 |
•
547/574 nm |
Fluorogenic substrates
These substrates do not require a quencher and contain a C-terminal dye that does not fluoresce
until it is cleaved from the peptide (fluorescent form of dye is released).
Dye (donor) | Donor Ex/Em | |
SensoLyte® AMC | AMC |
•
351/430 nm |
---|---|---|
SensoLyte® AFC | AFC |
•
382/480 nm |
SensoLyte® Rh110 | Rh110 |
•
501/527 nm |
Why is Protease Activity Important?
Protease enzymes catalyze the breakdown of proteins into smaller peptide fragments and/or single amino acids.
Endopeptidases are proteases that cleave internally of the protein sequence,
and those that cleave at the N-terminus or C-terminus are exopeptidases.
Endopeptidases are categorized into 4 classes according to the catalyst type (amino acid or metal)
that is located within the active site that drives the hydrolytic breakdown of the substrate peptide bond, including
(a)Serine Proteases (Trypsin, Elastin, Thrombin, HCV NS3/4A), (b)Cysteine proteases (Caspase, Cathepsin),
(c)Aspartyl Proteases (HIV, Renin), and (d) Metalloproteases (MMPs).
Through very specific and controlled protein substrate cleavage, proteases help in the initiation, modulation and termination
of many crucial cellular functions, including DNA replication, cell proliferation, wound healing, immunity, angiogenesis and apoptosis.
Proteases are involved in many diseases caused by aberrant metabolic protease activity or
through infection by pathogens that express proteases for their infectivity or replication.
Role of Proteases
Activation of Bid in apoptosis by Caspases-8, Calpains & Cathepsins B, L S, H, D, acting via the loops or interdomain linker regions.
Processing antigens via the lysosomal system for presentation by MHC class II molecules, such as cathepsins and deubiquitinating enzymes.
Ectodomain processors by exposing cryptic sites, such as beta-secretase during amyloid precursor protein processing or Shedding various transmembrane proteins such as metalloproteases and cysteine proteases.
ECM modulators by using exosites as effective substrate binding, such as thrombin, ADAMTS-4 and most MMPs.
Targets for viral inhibitors to arrest viral proteases processing viral polyproteins in causing pathogenicity