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Host: Rabbit Species Reactivity: Human, rat and mouse. Reactivity of Anti-Caspase 3 (p12) was confirmed by ELISA and dot blot. Specificity was confirmed by Western blot analysis in 3T3 cell lysate.Application: ELISA, WBProduct Description: This polyclonal Anti-Caspase 3 antibody is supplied as an epitope affinity purified rabbit IgG, 50 µg in 250 µl (0.2 mg/µl) of Phosphate Buffered Saline (pH 7.4) containing 0.05% Sodium Azide. Immunogen: Rabbit polyclonal Anti-Caspase (p12) antibody was raised against a synthetic peptide derived from the p12 domain of human Caspase 3 preproprotein (GenBank accession #: NP_004337).Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific application should be determined by the investigator.
ELISA for immunizing peptide: 1:5,000-20,000WB: 1:500-1:1,000IHC (recommended but not tested): 1:200