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Peptides  >  Enzyme Substrates and Inhibitors  >>  Mca-RPPGFSAFK(Dnp), fluorogenic

Product Name Mca - RPPGFSAFK(Dnp), fluorogenic
Mca - RPPGFSAF - K(Dnp)
Size 0.5 mg
Catalog # 63798
US$ $181
Purity % Peak Area By HPLC ≥ 95%
Description

This peptide is a fluorescence resonance energy transfer (FRET) substrate for assaying ECE-1 and ECE-2, ACE1 and ACE2. neprilysin, thermolysin (TLN), MMP-2 and MMP9, thimet oligopeptidase, cathepsin A, cathepsin X/Z, BACE, and insulinase, but not MMP1. Fluorescence of Mca (donor) is quenched by Dnp (quencher) in the intact peptide. Proteolytic cleavage of the FRET peptide separates the donor and the quencher, recovering the quenched fluorescence of Mca that is directly related to the enzyme activities and can be monitored at Ex/Em: 328nm/393nm.

Detailed Information Material Safety Data Sheets (MSDS)
Storage -20C
References Johnson, G.D. et al. Anal. Biochem. 286, 112 (2000); Mzhavia, N. et al. J. Biol. Chem. 278, 14704 (2003); Sigman, J.A. et al. Biochem. J. 388, 255 (2005);
Molecular Weight 1388.5
Sequence
(One-Letter Code)
Mca-RPPGFSAF-K(Dnp)
Sequence
(Three-Letter Code)
Mca - Arg - Pro - Pro - Gly - Phe - Ser - Ala - Phe - Lys(Dnp) - OH
     
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