SensoLyte® 390 Cathepsin E Assay Kit *Fluorimetric*NEW
Cathepsin E is a non-lysosomal aspartic proteinase of the pepsin superfamily. It plays an important role in the protein degradation, the generation of bioactive proteins, and antigen processing. Recent studies have particularly suggested that Cathepsin E is important in host defense against cancer cells and invading microorganisms. The SensoLyte® 390 Cathepsin E Assay Kit is a convenient assay for screening of enzyme inhibitors and for continuous assay of Cathepsin E activity using a Mca/Dnp FRET peptide. In the FRET peptide, the fluorescence of Mca is quenched by Dnp. Upon cleavage into two separate fragments by Cathepsin E, the fluorescence of Mca is recovered, and can be monitored at excitation/emission = 330 nm/390 nm. The assay can detect as low as 2 ng/mL active Cathepsin E. The kit contains: • Mca/Dnp-based FRET peptide substrate (Ex/Em=330/390 nm upon cleavage) • Assay buffer • Human recombinant Cathepsin E • Inhibitor • Fluorescence reference standard for calibration • A detailed protocol Kit size: 100 assays (96-well plate).
Figure 1. Inhibition of Cathepsin E activity by Pepstatin A as measured with SensoLyte® 390 Cathepsin E Assay Kit.