AnaSpec, EGT Group is pleased to offer a wide selection of SYBR® Green-based
MasterMixes and Core Kits for almost any thermocycler available in the industry.
Our MESA (Maximize & Enhance
your SYBR® Assays)
MasterMixes (see Tables 1-3) use our new powerful MeteorTaq hotstart
polymerase.
Our MESA BLUE kits contain a proprietary
inert BLUE dye that aids in the visualization of reagent dispensed, thus avoiding
aberrant results before they occur. Our CORE kits are unique in the industry
in that they are probably the only format in the industry that provide users
with the ability to titrate SYBR® Green amount as part of the optimization process.
AnaSpec, EGT Group’s qPCR MasterMixes:
Reduce run times with uncompromised results
• Tailored for highly sensitive assays
• Optimized for maximum consistency and specificity
• Large dynamic range
Available in BLUE format
• New proprietary inert BLUE
dye
• Clear visual dispensing of the reagent
To learn more about qPCR, please download the qPCR Guide by clicking
here.
Figure 1. Comparison between MESA GREEN qPCR MasterMix
Plus for SYBR® Assay Low ROX and Competitor S SYBR® Green kit. Experiment was performed using 18S primers
and the same cDNA on a Mx3000P®. MESA GREEN MasterMix showed better sensitivity, earlier
Cq (Ct) values, higher ΔRn and steeper amplification curves compared to
Competitor S SYBR® Green reagent.
Table 1. MESA FAST MasterMix Plus for SYBR® assay uses our new powerful MeteorTaq
hotstart polymerase which in combination with a newly formulated buffer results
in greater sensitivity. Kits are optimized for use in fast cycling mode but
can also be used in regular cycling mode.
| Product |
Size |
Catalog # |
| MESA
FAST qPCR MasterMix Plus for SYBR® Assay No ROX
- compatible for use in
FAST cycling conditions on LC480 and Smartcyclers and RotorGene 6000. |
600 RXNs |
RT-SY2X-03+NRWOUF |
| MESA
FAST qPCR MasterMix Plus dTTP for SYBR® Assay No ROX
- compatible for use in
FAST cycling conditions on LC480, Smartcyclers and RotorGenes 6000/Q,
and in regular cycling conditions on Mx4000®, Mx3000P®, Mx3005P®, MasterCycler®
ep realplex, RotorGene 2000/3000/6000/Q, CFX96/384, DNA Engine Opticon®
1, Opticon® 2, Chromo 4, Mini Opticon®, Quantica® and LC480. |
600 RXNs |
RT-SY2X-03+NRWOUNF |
| MESA
FAST qPCR MasterMix Plus for SYBR® Assay Low ROX
- compatible
for use in FAST cycling conditions on ABI Prism®
7500. |
600 RXNs |
RT-SY2X-03+WOULRF |
| MESA
FAST qPCR MasterMix Plus for SYBR® Assay ROX
- compatible for use in
FAST cycling conditions on ABI Prism® 7900, StepOne™ and MasterCycler®
ep realplex. |
600 RXNs |
RT-SY2X-03+WOUF |
Table 2. MESA Green qPCR MasterMix Plus also uses our powerful MeteorTaq
hotstart polymerase for greater sensitivity. Kits are optimized for use on regular
cycling mode but can also be used in fast cycling mode.
| Product |
Size |
Catalog # |
| MESA
GREEN qPCR MasterMix Plus for SYBR® Assay No ROX
- compatible for use on
Mx4000®, Mx3000P®, Mx3005P®, Engine Opticon® 1 & 2, Chromo 4, Mini
Opticon®, RotorGene 2000, 3000, 6000, Quantica®, and LC 480. |
600 RXNs
1200 RXNs
4000 RXNs |
RT-SY2X-03+NRWOU
RT-SY2X-06+NRWOU
RT-SY2X-20+NRWOU |
| MESA
GREEN qPCR MasterMix Plus for SYBR® Assay No ROX + fluorescein
- fluorescein is needed
to normalize the data correctly when using iCycler iQ®, iQ5 or My iQ®
thermocyclers. This kit can be used on these machines. |
600 RXNs
1200 RXNs
4000 RXNs
5 x 600 RXNs |
RT-SY2X-03+WOUFL
RT-SY2X-06+WOUFL
RT-SY2X-20+WOUFL
05-SY2X-03+WOUFL |
| MESA
GREEN qPCR MasterMix Plus for SYBR® Assay Low ROX
- compatible
for use on ABI 7500, Mx4000®, Mx3000P® and Mx3005P®. |
600 RXNs
1200 RXNs
4000 RXNs |
RT-SY2X-03+WOULR
RT-SY2X-06+WOULR
RT-SY2X-20+WOULR |
| MESA
GREEN qPCR MasterMix Plus dTTP for SYBR® Assay ROX
- compatible
for use on ABI prism® 5700, 7000, 7700, 7900, 7300,
StepOne™ and MasterCycler® ep realplex. |
600 RXNs |
RT-SY2X-03+WOUN |
| MESA
GREEN qPCR MasterMix Plus for SYBR® Assay ROX
- compatible
for use on ABI prism® 5700, 7000, 7700, 7900, 7300,
StepOne™ and MasterCycler® ep realplex. |
600 RXNs
1200 RXNs
4000 RXNs |
RT-SY2X-03+WOU
RT-SY2X-06+WOU
RT-SY2X-20+WOU |
| One
step MESA GREEN qPCR MasterMix Plus for SYBR® Assay No ROX
- compatible for use on
Mx4000®, Mx3000P®, Mx3005P®, DNA Engine Opticon® 1 & 2, Chromo 4,
CFX96, Mini Opticon®, RotorGene 2000, 3000, 6000, Quantica®, and LC 480. |
600 RXNs |
RT-SYRT-032XNR |
| One
step MESA GREEN qPCR MasterMix Plus for SYBR® Assay ROX
- compatible for use on
ABI prism® 5700, 7000, 7700, 7900, 7300, StepOne™ and MasterCycler® ep
realplex. |
600 RXNs |
RT-SYRT-032X |
Table 3. MESA BLUE qPCR MasterMix Plus for SYBR® Assay uses our new powerful
MeteorTaq hotstart polymerase in combination with a newly formulated
buffer. A new proprietary inert Blue dye for visualization purpose that does
not affect reaction is also included. These result in greater sensitivity and
ease of use. Kits are optimized for use on fast cycling mode but can also be
used in regular cycling mode.
| Product |
Size |
Catalog # |
| MESA
BLUE qPCR MasterMix Plus for SYBR® Assay No ROX
- compatible for use on
Mx4000®, Mx3000P®, Mx3005P®, Engine Opticon® 1 & 2, Chromo 4, Mini
Opticon®, RotorGene 2000, 3000, 6000, Quantica®, and LC 480. |
600 RXNs |
RT-SY2X-03+NRWOUB |
| MESA
BLUE qPCR MasterMix Plus for SYBR® Assay No ROX + fluorescein
-fluorescein is needed to
normalize the data correctly when using iCycler iQ®, iQ5 or My iQ® thermocyclers.
This kit can be used on these machines. |
600 RXNs |
RT-SY2X-03+WOUFLB |
| MESA
BLUE qPCR MasterMix Plus for SYBR® Assay, Low ROX
- compatible
for use on ABI 7500, Mx4000®, Mx3000P® and Mx3005P®. |
600 RXNs |
RT-SY2X-03+WOULRB |
| MESA
BLUE qPCR MasterMix Plus for SYBR® Assay ROX
- compatible
for use on ABI Prism® 5700, 7000, 7700, 7900, 7300,
StepOne™ and MasterCycler® ep realplex. |
600 RXNs |
RT-SY2X-03+WOUB |
Table 4. qPCR Core kits and MasterMix Plus for SYBR® Green I use the hotstart
enzyme, HotGoldStar. These kits can be adapted for use on 1 or 2-step reaction.
| Product |
Size |
Catalog # |
| qPCR
Core kit for SYBR® Assay No ROX
- compatible
for use on Mx4000®, Mx3000P®, Mx3005P®, MasterCycler® ep realplex, iCycler
iQ®, My iQ®, iQ5, DNA Engine Opticon® 1, DNA Engine Opticon® 2, Chromo
4, Mini Opticon®, Quantica®, LC480. |
1000 RXNs |
RT-SN10-05NR |
| qPCR
Core kit for SYBR® Assay ROX
- compatible
for use on ABI prism® 5700, 7000, 7700, 7900, 7300,
StepOne™ and MasterCycler® ep realplex. |
1000 RXNs |
RT-SN10-05 |
| qPCR
Core kit for SYBR® Assay dTTP ROX
- compatible
for use on ABI prism® 5700, 7000, 7700, 7900, 7300,
StepOne™ and MasterCycler® ep realplex. |
1000 RXNs |
RT-SN10-05NU |
| qPCR
Core kit for SYBR® Assay Only dUTP ROX
- compatible
for use on ABI prism® 5700, 7000, 7700, 7900, 7300,
StepOne™ and MasterCycler® ep realplex. |
1000 RXNs |
RT-SN10-05OU |
| qPCR
MasterMix for SYBR® Assay ROX
- compatible
for use on ABI prism® 5700, 7000, 7700, 7900, 7300,
StepOne™ and MasterCycler® ep realplex. |
600 RXNs |
RT-SN2X-03T |
| qPCR
MasterMix Plus for SYBR® Assay No ROX
- compatible for use on
Mx4000®, Mx3000P®, Mx3005P®, Engine Opticon® 1 & 2, Chromo 4, Mini
Opticon®, RotorGene 2000, 3000, 6000, Quantica®, and LC 480. |
600 RXNs |
RT-SN2X-03+NR |
| qPCR
MasterMix Plus for SYBR® Assay No ROX + fluorescein
-fluorescein is needed to
normalize the data correctly when using iCycler iQ®, iQ5 or My iQ® thermocyclers.
Alternatively a separate tube of fluorescein is available in case you
would like to use fluorescein in combination with any of our No ROX SYBR®
Green I kits. This kit can be used on these machines. |
600 RXNs |
RT-SN2X-03+NRFL |
| qPCR
MasterMix Plus for SYBR® Assay ROX
- compatible
for use on ABI prism® 5700, 7000, 7700, 7900, 7300,
StepOne™ and MasterCycler® ep realplex. |
600 RXNs
1200 RXNs
4000 RXNs |
RT-SN2X-03+
RT-SN2X-06+
RT-SN2X-20+ |
| qPCR
MasterMix Plus dTTP for SYBR® Assay ROX
- compatible
for use on ABI prism® 5700, 7000, 7700, 7900, 7300,
StepOne™ and MasterCycler® ep realplex. |
600 RXNs |
RT-SN2X-03+WOUN |
| qPCR
MasterMix Plus w/o UNG for SYBR® Assay Low ROX
- compatible
for use on ABI 7500, Mx4000®, Mx3000P®, Mx3005P®. |
600 RXNs |
RT-SN2X-03+WOULR |
| qPCR
MasterMix Plus w/o UNG for SYBR® Assay ROX
- compatible
for use on ABI prism® 5700, 7000, 7700, 7900, 7300,
StepOne™ and MasterCycler® ep realplex. |
600 RXs |
RT-SN2X-03WOU+ |
Click here
to download the Manual for qPCR MasterMix Plus, qPCR Mastermix and qPCR Core
Kit.
2012 Product Citations
MESA FAST for SYBR® Assays
Zong,
Y. et al. PNAS 109, E3395 (2012).
Moreau,
M. et al. Plant Cell 24, 463 (2012).
MESA Green for SYBR® Assays
Soto-Rifo,
R. et al. Nucleic Acids Res 40, 2653 (2012).
Cobo,
ER. et al. Infect Immun 80, 143 (2012).
Steinsiek,
S. K. Bettenbrock, J Bacteriol 194, 5897 (2012).
MESA BLUE for SYBR® Assays
Rast,
MI. and R. Simon, Plant Cell 24, 2917 (2012).
Bosch,
D. and A. Saiardi, J Biol Chem 287, 38347 (2012).
Fichelson,
P. et al. PNAS 109, 7893 (2012).
Core Kits
Zhang,
L. et al. Plant Physiol 160, 1453 (2012).
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