pleased to announce the release of two keratin antibodies that recognize both
the intact and caspase digested N-terminal of K18 and K19 at Asp237. These antibodies
are useful tools for studying keratin dynamics in mice undergoing hepatocyte
apoptosis, patients with cirrhosis, and also in apoptotic cells of various epithelial
human tumors in a non-invasive manner.
filaments (IFs), together with actin microfilaments and tubulin microtubules,
comprise the three major cytoskeletal networks that are found in most eukaryotic
cells.1 Keratins, which are the largest IF protein subgroup, can
be divided into the acidic type I (K9-K20) and basic type II (K1-K8) Keratins.2
Typically, epithelial cells express at least one of each type, and the proteins
exist in the cells as noncovalent obligate heteropolymers. Keratin networks
are highly dynamic and reorganized during cell differentiation, mitosis and
apoptosis, and also play important cytoprotective and structural support roles
for the cell.3,4 During apoptosis, the epithelial keratins 18 and
19 undergo caspase-mediated proteolysis, leading to the disassembly of the nuclear
envelope and cytoplasmic IF networks.5 K18 and K19 undergo caspase-cleavage
at the conserved Asp237 in their rod domains.5,6 In contrast to
other type I keratins, K18 is unique in that it includes another caspase-cleavage
site at Asp396 of the carboxy-terminal domain.7
Rabbit anti-human Keratin-18 (Asp237) or anti-human Keratin-19 (Asp237)
polyclonal antibodies were raised against a synthetic peptide corresponding
to an internal sequence surrounding amino acid 237 (GLTVEVD) of human Keratin-18
or amino acid 237 (QVSVEVD) of human Keratin-19, respectively.
Anti-Keratin 18 reacts to both cleaved keratin-18 (Asp237) (29 kDa)
and intact Keratin-18 (49 kDa) proteins from anisomycin-treated human colonic
carcinoma HT29 cell lysate and Fas antibody-induced mouse liver lysate (see
figure below).6 This antibody also reacts to cleaved Keratin-19 (Asp237)
(29 kDa).6 Anti-Keratin 19 reacts to both cleaved keratin-19 (Asp237)
(29 kDa) and intact Keratin-19 (45 kDa) proteins from anisomycin-treated human
colonic carcinoma HT29 cell lysate.6 This antibody also reacts to
cleaved Keratin-18 (Asp237) (29 kDa).6 Both antibodies are human,
mouse, and monkey reactive.
|| Anti-K-19 (asp237)
here to request AnaSpec’s latest Antibodies brochure
to view the abstract of a paper on anti-K18 and K-19 that recognize only the
cleaved K18/K19 Asp 237 antibodies published by Stanford University and AnaSpec
1. Ku, NO.
et al. Am J. Physiol. 277, G1108 (1999).
J. et al. J. Cell Biol. 174, 169 (2006).
MB. et al. Trends Biochem. Sci. 31, 383 (2006).
4. Omary, MB.
et al. N. Engl. J. Med. 351, 2087 (2004)
5. Ku, NO. et
al. J. Biol. Chem. 272, 33197 (1997).
6. Tao, GZ. et
al. J. Pathology. 215, 164 (2008).
7. Ku, NO. and
Omary, MB. J. Biol. Chem. 276, 26792 (2001).