AnaTag™ APC, B-PE and R-PE Labeling Kits – New & Improved


AnaSpec is pleased to announce the release of the improved AnaTag™ APC, B-PE and R-PE Labeling Kits.

Labeling of antibodies can be both tedious and time-consuming. Without the use of proper techniques and reagents, the conjugation may result in suboptimal or no labeling. AnaSpec’s improved APC, B-PE and R-PE Labeling Kits contain all the reagents needed for optimized labeling of antibodies. The phycobiliproteins, modified by SMCC, result in the creation of SH-reactive moieties, which can easily react with the thiol groups of target antibody. Since no additional activation is required, the conjugation process is simple and easy.

Phycobiliproteins are a family of highly soluble and fluorescent proteins derived from cyanobacteria and eukaryotic algae. Allophycocyanin (APC) is an ultra-sensitive, near-infrared fluorescent tracer with a high quantum yield (Ex/Em = 650 nm/660 nm). B-Phycoerythrin (B-PE), a fluorescent protein from phycobiliprotein family, has a primary absorption peak at 545 nm with a secondary peak at 563 nm and maximum emission at 578 nm. R-Phycoerythrin (R-PE), made of a, b and g subunits and present as (ab)6g, has broad absorption bands with peaks at 565 nm (eM =1.96 X 106 M-1cm-1), 498 (eM =1.53 X 106 M-1cm-1), and 539 nm (eM =1.62 X 106 M-1cm-1. These phycobiliproteins are significantly more sensitive and photostable than conventional organic fluorophores. APC, B-PE or R-PE labeled antibodies are used in applications such as flow cytometry and immunofluorescence.

AnaTag kits provide all the reagents, purification column needed to label up to 1 mg of antibody. A detailed step-by-step protocol is also included. APC, B-PE or R-PE and SMCC activated derivatives of these proteins can also be purchased separately.

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Figure 1.
Tubulin in bovine pulmonary artery endothelial cells were detected with mouse anti-alpha-tubulin primary antibody, visualized with APC-conjugated goat anti mouse IgG. Nuclei were stained with DAPI (cat# 83210)


Figure 2.
Actins in bovine pulmonary artery endothelial cells were detected with biotin-conjugated phalloidin, visualized with R-PE-conjugated streptavidin (cat#60669). Nuclei were stained with Hoechst 33342 (cat# 83218).

Protein Labeling Kits

Product

Ex/Em (nm/nm)

Size

Cat #

AnaTag™ APC Labeling Kit NEW

650/660

1 kit

72111

AnaTag™ B-PE Labeling Kit NEW

545, 563/578

1 kit

72112

AnaTag™ R-PE Labeling Kit NEW

565, 498, 539/578

1 kit

72113


APC, B-PE and R-PE Products


Product

Size

Cat #

B - PE (B - Phycoerythrin)

1 mg

82001

CL - APC (Cross Linked - Allophycocyanin)

1 mg

82002

R - PE (R - Phycoerythrin)

1 mg

82004

SMCC Activated B - PE (B - Phycoerythrin) NEW

1 mg

72109

SMCC Activated CL - APC (Cross Linked - Allophycocyanin) NEW

1 mg

72108

SMCC Activated R - PE (R - Phycoerythrin) NEW

1 mg

72110











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